小麦热激蛋白转录因子C家族基因的结构及其在干旱胁迫中的表达  

Structure and Expression of Heat Shock Protein Factor C Genes Subfamily Response to Drought in Wheat

作　　者：王付娟 李宗震 仝胜利 李淑梅 WANG Fujuan;LI Zongzhen;TONG Shengli;LI Shumei(College of Agronormy,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000,China;College of Agronomy,Henan Agricultural University,Zhengzhou,Henan 450002,China)

机构地区：[1]信阳农林学院农学院,河南信阳464000 [2]河南农业大学农学院,河南郑州450002

出　　处：《麦类作物学报》2020年第4期416-424,共9页Journal of Triticeae Crops

摘　　要：小麦热激蛋白转录因子在热胁迫和耐热性产生的过程中发挥重要作用。为进一步了解小麦热激蛋白转录因子C亚家族,本研究采用全基因组信息保守结构域比对和进化聚类分析,共鉴定了24个小麦TaHsfC基因,并对TaHsfC亚家族成员染色体定位、基因结构、亚细胞定位、对应蛋白质的氨基酸特点、基因表达进行了分析。结果表明,24个TaHsfCs主要分布在5条染色体上,其中A基因组中有7个,B基因组中9个,D基因组中有6个,另外两个所在染色体位置不清楚。TaHsfCs含有0~2个内含子,其中TaHsfC1亚族基因含有1~2个内含子,TaHsfC2亚族基因则含有0~1个内含子。聚类分析表明,小麦TaHsfC成员共分为TaHsfC1和TaHsfC2两个亚族。24个TaHsfCs成员全部定位于细胞核。在15%PEG模拟干旱条件下,干旱敏感品种矮抗58和耐旱品种晋麦47中有10个TaHsfC基因显著上调表达,其中TaHsfC2亚族基因的表达量上调倍数均高于TaHsfC1亚族基因(其中TaHsfC2j和TaHsfC2k未在晋麦47中检测到表达量)。本研究可为探索小麦热激蛋白转录因子C家族基因在小麦抗旱中的分子机制提供一定的理论支撑。Heat shock protein transcription factors(HSFs)play an important role in the production of heat stress and heat resistance.Many heat shock protein transcription factors are up-regulated under drought and salt stress.HSFs are divided into three subfamilies:A,B,and C,but the function of members in the heat shock protein transcription factor C subfamily(TaHsfC)has not been fully validated in any species.In order to systematically analyze the wheat TaHsfC subfamily,this study used whole-genome information conserved domain alignment and evolutionary clustering analysis to identify a total of 24 wheat TaHsfC genes,and analyzed the amino acid characteristics of the corresponding proteins by chromosomal localization of TaHsfC subfamily members.The results showed that 24 TaHsfCs were mainly distributed on five chromosomes,with seven in A genome,nine in B genome and six in D genome.The other two chromosomes were unknown.Cluster analysis showed that TaHsfC members were divided into two subgroups TaHsfC1 and TaHsfC2.There were zero to two introns in the TaHsfCs,with one or two introns in TaHsfC1 and zero or one intron in TaHsfC2.All 24 TaHsfCs were located in nucleus.Results of gene expression data showed that 10 TaHsfC genes were significantly up-regulated in Aikang 58 and Jinmai 47 cultivars,and the expression level of TaHsfC2 genes was higher than that of TaHsfC1 genes(TaHsfC2j and TaHsfC2k were not detected in Jinmai 47).The comprehensive analysis of TaHsfCs can provide some theoretical support for drought tolerance breeding in wheat.

关 键 词：热激蛋白转录因子C 干旱胁迫 基因结构分析 表达模式分析 小麦 

分 类 号：S512.1[农业科学—作物学] S330