纳米无机载银磷酸锆树脂基托对6种口腔细菌的体外抗菌研究  

Antimicrobial Activity of Resin Base with Nano-silver Inorganic Zirconium Phosphate against Six Oral Bacteria in Vitro

作　　者：杨成雪 翟羽翔 王开梅 陈林 YANG Chengxue;ZHAI Yuxiang;WANG Kaimei;CHEN Lin(Stomatological Hospital Affiliated to Zunyi Medical College, Zunyi 563000, China)

机构地区：[1]遵义医科大学附属口腔医院,贵州遵义563003

出　　处：《口腔医学研究》2020年第3期243-246,共4页Journal of Oral Science Research

基　　金：贵州省科技技术厅联合基金项目(Ck-836)号;贵州省遵义市红花岗科技局项目2019(26)号;遵义医学院硕士启动基金KY2017-05号。

摘　　要：目的:通过体外粘附实验,研究纳米无机载银磷酸锆加入到树脂基托中后是否具有抗菌性。方法:将纳米无机载银磷酸锆(Conval PAg-40型)按1%、3%、5%、7%比例添加到树脂基托中制成树脂片,未添加者为对照组,打磨抛光,金相显微镜观察试件表面无划痕、粗糙度测定仪测得表面粗糙度值无统计学差异后,采用薄膜密贴法检测该抗菌树脂片对变形链球菌、黏性放线菌、内氏放线菌、血链球菌、牙龈卟啉单胞菌、白色念珠菌的抗菌性,比较分析树脂基托抗菌性能与添加抗菌剂比例的相关性。结果:3%、5%、7%组培养的牙龈卟啉单胞菌以及1%、3%、5%、7%组培养的变形链球菌、黏性放线菌、内氏放线菌、血链球菌、白色念珠菌菌落数均低于对照组,差异有统计学意义(P<0.05),抗菌率随添加比例的增加逐渐升高;1%组培养的牙龈卟啉单胞菌菌落数与对照组相比差异无统计学意义(P>0.05)。结论:纳米无机载银磷酸锆树脂基托有良好的抗菌效能,其抗菌率随抗菌剂比例的添加而呈线性上升趋势。Objective:To study whether the nano-silver inorganic zirconium phosphate has antibacterial properties after being added to resin base by adhesion experiments in vitro.Methods:The nano-silver inorganic zirconium phosphate(Conval PAg-40)was added to the resin base at the ratio of 1%,3%,5%,and 7%to prepare resin specimens.The antimicrobial properties of all resin specimens against Streptococcus mutans,Actinomyces viscosus,Actinomyces naeslundii,Streptococcus sanguis,Porphynomoans gingivalis,and Candida albicans were tested by the film-sticking method after those specimens were milled and polished.The surface roughness values were measured by the roughness tester.Results:The colonies of Porphyromonas gingivalis cultured in groups of 3%,5%,and 7%,and Streptococcus mutans,Actinomyces viscosus,Actinomyces naeslundii,Streptococcus sanguis,and Candida albicans cultured in groups of 1%,3%,5%,and 7%were lower than the control group(P<0.05).The antimicrobial rate of resin specimens gradually increased with the increase of addition.There was no significant difference in the number of Porphyromonas gingivalis colonies cultured in 1%group compared with that of the control group(P>0.05).Conclusion:The resin base with nano-silver inorganic zirconium phosphate has good antimicrobial activity,and its antimicrobial rate increases linearly with the proportion of antimicrobial agents.

关 键 词：义齿树脂基托 纳米无机载银磷酸锆 抗菌 

分 类 号：R78[医药卫生—口腔医学]