干旱-低温交叉适应性对茶树抗寒性的影响  

Effect of cross adaptation of drought-low temperature on cold resistance of tea plants(Camellia sinensis)

作　　者：徐义群 胡哲 胡月朦 江昌俊[1] 李娟 李叶云[1] XU Yiqun;HU Zhe;HU Yuemeng;JIANG Changjun;LI Juan;LI Yeyun(State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei 230036;Biotechnology Center,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学茶树生物学与资源利用国家重点实验室,合肥230036 [2]安徽农业大学生物技术中心,合肥230036

出　　处：《安徽农业大学学报》2020年第1期1-6,共6页Journal of Anhui Agricultural University

基　　金：安徽农业大学茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20160111);安徽农业大学省级大学生创新创业训练计划项目(201810364146)共同资助。

摘　　要：为了解干旱-低温交叉适应对茶树抗寒性的影响,以一年生无性系品种舒茶早茶苗为试验材料,用20%聚乙二醇6000(PEG-6000)模拟干旱预处理,测定低温胁迫过程中茶树半致死温度、丙二醛、抗氧化酶活性、渗透物质和内源激素等部分生理指标的变化。结果表明,经过PEG-6000预处理的茶苗半致死温度LT50明显下降。低温条件下,茶苗叶片丙二醛、抗氧化酶活性、渗透物质和激素含量整体呈上升趋势。低温胁迫第7天时,与对照相比,PEG-6000预处理的茶苗叶片丙二醛含量下降44.6%,SOD、POD酶活分别升高78%和25%,可溶性蛋白、溶性糖含量分别增加44.6%及20.0%;内源激素ABA与SA含量分别提高97.7%和122.0%。干旱可诱导茶树对低温胁迫的交叉适应性,这种交叉适应性与茶树的抗氧化酶活性、渗透物质和内源激素的调节能力有关。In order to find out the effect of drought-low temperature cross adaptation on the cold resistance of tea plants, tea seedlings of annual ‘Shuchazao’ [Camellia sinensis(L.)O. Kuntze] were used as materials to carry out the experiment. Tea seedlings were pretreated under drought condition, which simulated with 20% polyethylene glycol-6000(PEG-6000), to determine the changes of some physiological indexes such as semi-lethal temperature, malondialdehyde, antioxidant enzyme activity, osmotic substances and endogenous hormones during low temperature stress. The results showed that the semi-lethal temperature LT50 of the tea seedlings pretreated with PEG-6000 decreased significantly. Under low temperature, the content of MDA, antioxidant enzyme activities, osmotic substances and hormones in the tea seedlings leaves increased as a whole. On the 7 th day of low temperature stress, compared with the control, the MDA content in the tea seedlings leaves pretreated with PEG-6000 decreased by 44.6%, SOD and POD activities increased by 78% and 25%, the contents of soluble protein and soluble sugar increased by 44.6% and 20.0%, and the contents of endogenous hormones ABA and SA increased by 97.7% and 122.0%, respectively. Drought can induce cross-adaptability of tea plants to low temperature stress, which is related to the regulation of antioxidant enzymes, osmotic substances and endogenous hormones.

关 键 词：茶树 低温胁迫 交叉适应性 抗氧化酶 渗透物质 内源激素 

分 类 号：S571.1[农业科学—茶叶生产加工]