虎杖提取物对人胰腺癌细胞系Panc-1增殖与凋亡的影响  

Effects of Polygonum Cuspidate Extract on the Proliferation and Apoptosis of Human Pancreatic Cancer Cell Line Panc-1

作　　者：赵静[1] 杨兴武 王旗 王亮 王国泰 嵇文波 陈国仓 王鑫 ZHAO Jing;YANG Xing-wu;WANG Qi;WANG Liang;WANG Guo-tai;JIWen-bo;CHEN Guo-cang;WANG Xin(Shaanxi University of Chinese Medicine,Xianyang,Shaanxi,712000,China;Affiliated Hospital of Shaanxi University of Chinese Medicine,Hepatological Surgery,Xianyang,Shaanxi,712000,China)

机构地区：[1]陕西中医药大学,陕西咸阳712000 [2]陕西中医药大学附属医院肝胆外科,陕西咸阳712000

出　　处：《现代生物医学进展》2020年第1期50-54,58,共6页Progress in Modern Biomedicine

基　　金：咸阳市科技局科研项目(2018KT-23)。

摘　　要：目的:通过虎杖提取物干预人胰腺癌细胞系Panc-1,探讨虎杖提取物对人胰腺癌细胞增殖凋亡表型的影响。方法:制备不同浓度(0、10、50、100、150、200μg/m L)的虎杖提取物,将各个浓度的虎杖提取物分别加入待处理的人胰腺癌Panc-1细胞系中持续培养24 h后,利用CCK-8(cell counting kit-8)法检测细胞株Panc-1的增殖活性;将100μg/m L虎杖提取物处理人胰腺癌细胞系Panc-124 h后,利用流式细胞术(FCM)检测其细胞周期及凋亡分布;100μg/m L虎杖提取物处理人胰腺癌细胞株Panc-124 h后,提取细胞总RNA及总蛋白,后续利用实时荧光定量PCR及Western blot分别检测人胰腺癌细胞株Panc-1增殖标志基因PCNA、CDK2及凋亡标志基因BAD、BAX的转录和翻译水平。结果:CCK-8结果表明虎杖提取物对人胰腺癌细胞系Panc-1细胞增殖的抑制率随浓度增加;流式细胞术结果显示虎杖提取物抑制人胰腺癌细胞增殖促进其凋亡;荧光定量PCR和Western blot结果显示虎杖提取物能使人胰腺癌细胞增殖标志基因PCNA,CDK2表达量下降,凋亡标志基因BAD,BAX表达量上升。结论:虎杖提取物能够抑制人胰腺癌细胞系Panc-1细胞增殖并促进其凋亡。Objective:The effect of Polygonum cuspidatum extract on Pancreatic cancer cell line Panc-1 was investigated by extracting Polygonum cuspidatum extracts into human pancreatic cancer cell proliferation and apoptosis phenotype.Methods:Different concentrations(0,10,50,100,150,200μg/m L)of Polygonum cuspidatum extract were prepared,and each concentration of Polygonum cuspidatum extract was added to the human pancreatic cancer Panc-1 cell line to be treated for 24 h.The proliferation activity of the cell line Panc-1 was detected by CCK-8(cell counting kit-8)method;the human pancreatic cancer cell line Panc-1 was treated with 100μg/m L Polygonum cuspidatum extract for 24 h,and then flow cytometry was used(FCM)was used to detect the cell cycle and apoptosis distribution;100μg/m L Polygonum cuspidatum extract was used to treat human pancreatic cancer cell line Panc-1 for 24 h,and the total RNA and total protein were extracted.Subsequent real-time PCR and Western blot were used to detect human pancreatic cancer.Transcription and translation levels of the cell line Panc-1 proliferation marker genes PCNA,CDK2 and apoptosis marker genes BAD,BAX.Results:The results of CCK-8 showed that the inhibition rate of Polygonum cuspidatum extract on the proliferation of human pancreatic cancer cell line Panc-1 increased with concentration.The results of flow cytometry showed that Polygonum cuspidatum extract inhibited the proliferation of human pancreatic cancer cells and promoted its apoptosis.The results of real-time PCR and Western blot showed The extract of Polygonum cuspidatum can decrease the expression of PCNA and CDK2 in human pancreatic cancer cell proliferation,and the expression of apoptosis marker gene BAD and BAX increased.Conclusion:Polygonum cuspidatum extract can inhibit the proliferation and promote apoptosis of human pancreatic cancer cell line Panc-1.

关 键 词：虎杖 胰腺癌 PANC-1 细胞增殖 细胞凋亡 

分 类 号：R-33[医药卫生] R735.9