IL-10在输血相关性移植物抗宿主病小鼠模型中的免疫调节作用  

Immunomodulatory Effect of IL-10 in Transfusion-associated Graft-versus-host Disease Mouse Model

作　　者：梁静[1] 凯赛尔江·多来提 范娜[1] 刘雯[1] 古丽美热·艾买如拉[1] 郑淑贤[1] LIANG Jing;KAIsaierjiang·duolaiti;FAN Na;LIU Wen;GU Li mei re·ai mai ru la;ZHENG Shu-xian(Blood transfusion branch,The sixth affiliated hospital of xinjiang medical university,Urumqi,Xinjiang,830002,China;Human anatomy department of preclinical college of xinjiang medical university,Urumqi,Xinjiang,830011,China)

机构地区：[1]新疆医科大学第六附属医院,新疆乌鲁木齐830002 [2]新疆医科大学基础医学院人体解剖学教研室,新疆乌鲁木齐830011

出　　处：《现代生物医学进展》2020年第1期55-58,共4页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区自然科学基金项目(2016D01C214)。

摘　　要：目的:IL-10在输血相关性移植物抗宿主病小鼠模型中的免疫调节作用。方法:取BALB/c实验小鼠免疫活性淋巴细胞,分别输注于BALB/c小鼠(设为A组)及BALB/c裸鼠(设为B组),建立TA-GVHD模型,观察小鼠症状,HE染色判断小鼠肝、肺、小肠、皮肤病理变化情况;采用双夹心酶联免疫吸附法(ELISA)检测两组小鼠血清IL-10浓度;用逆转录聚合酶链反应法RT-PCR检测移植后外周血单个核细胞中IL-10的表达。结果:A组中2只死亡(12.5%),B组中3只死亡(18.75%),共5只死亡,29只存活,两组死亡率比较无明显差异(P>0.05)。B组小鼠累及肝、肺、小肠和皮肤病理损伤程度较A组严重;存活小鼠IL-10浓度较死亡小鼠明显升高(P2<0.05);存活小鼠IL-10 mRNA表达阳性率96.55%明显高于死亡小鼠(20.00%)。结论:IL-10在输血相关的移植物抗宿主病小鼠模型中发挥负向免疫调节--免疫抑制作用。Objective:To investigate the immunomodulatory effect of IL-10 on mice with transfusion-associated graft-versus-host disease(TA-GVHD).Methods:The immune active lymphocytes of BALB/c experimental mice were infused into BALB/c mice(group A)and BALB/c nude mice(group B),respectively.the TA-GVHD model was established and the symptoms of mice were observed.HE staining was used to determine the pathological changes of liver,lung,small intestine and skin of mice.The concentration of serum IL-10 in the two groups was detected by double sandwich enzyme-linked immunosorbent assay(ELISA),and the expression of IL-10 in peripheral blood mononuclear cells(PBMCs)after transplantation was detected by reverse transcriptase polymerase chain reaction(RT-PCR).Results:Two rats died in group A(12.5%),B group,3 died(18.75%),5 died and 29 survived.there was no significant difference in mortality between the two groups(P>0.05).The pathological damage of liver,lung,small intestine and skin in group B was more serious than that in group A;The concentration of IL-10 in surviving mice was significantly higher than that in dead mice(P<0.05),and the positive rate of IL-10 mRNA expression in surviving mice was 96.55%,which was significantly higher than that in dead mice(20.00%).Conclusion:IL-10 may play an immunosuppressive role in the mouse model of TA-GVHD.

关 键 词：IL-10 输血相关性移植物抗宿主病 免疫抑制 

分 类 号：R-33[医药卫生] R457.2