分子生物学在血液病诊断和治疗中的应用及研究进展  

Research Progress on the Application of Molecular Biology in Hematological Diseases

作　　者：史美玉 唐诗哲 徐挺亮 周凯燕 周洪波[1,2] SHI Mei-yu;TANG Shi-zhe;XU Ting-liang;ZHOU Kai-yan;ZHOU Hong-bo(School of Minerals Processing and Bioengineering,Central South University,Changsha,Hunan,410083,China;Key Laboratory of Biometallurgy,Ministry of Education,Central South University,Changsha,Hunan,410083,China)

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083 [2]中南大学生物冶金教育部重点实验室,湖南长沙410083

出　　处：《现代生物医学进展》2020年第4期797-800,775,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31870115);湖南省自然科学省市联合基金资助项目(15JJ5006);中南大学中央高校基本科研业务费专项资金资助项目(2015zzts268)。

摘　　要：分子生物学自问世以来在各个领域都有着非常广泛的应用。随着分子生物学手段的不断发展,其在医学诊断和疾病治疗等领域的作用也获得医护人员的认可。分子生物学方法在血液病诊断和治疗方面的应用也尤为突出。本文综述了PCR,荧光原位杂交技术、基因芯片和二代测序等分子生物学手段在血液病中的应用,为血液病的诊断、治疗监测以及用药指导等方面的研究提供参考。Molecular biology has been widely used in many fields. With the continuous development of molecular biology, its role in medical diagnosis and disease treatment has been recognized by medical staff. The application of molecular biological methods in the diagnosis and treatment of hematological diseases is particularly prominent. The application of molecular biological methods such as PCR, fluorescence in situ hybridization, gene microarray and second-generation sequencing in hematological diseases were reviewed in this paper.

关 键 词：PCR 荧光原位杂交技术 基因芯片 二代测序 白血病 淋巴瘤 

分 类 号：Q75[生物学—分子生物学] R-33[医药卫生]