NF-κB介导的连翘酯苷B对宫颈癌细胞增殖活性的抑制作用  

Forsythiaside B inhibits the proliferation activity of HeLa cells though transcription factor NF-κB

作　　者：张哲 吕毓 黄攀豪[2] ZHANG Zhe;LYU Yu;HUANG Panhao(Reproductive Center of Hunan Provincial People's Hospital/the First Affiliated Hospital of Hunan Normal University,Changsha 410005,Hunan,China;Department of Pharmacy,the Third XiangYa Hospital of Central South University,Changsha 410078,Hunan,China)

机构地区：[1]湖南省人民医院/湖南师范大学附属第一医院生殖医学中心,湖南长沙410005 [2]中南大学湘雅三医院药学部,湖南长沙410078

出　　处：《中国临床药理学与治疗学》2020年第4期387-392,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖南省自然科学基金项目(2019JJ80032);湖南省卫生计生委科研计划课题项目(B20180820);长沙市科技局基础研究项目(kq1907064)。

摘　　要：目的:研究连翘酯苷B是否通过抑制NF-κB调控p21/Cyclin E/CDK2信号通路,对宫颈癌细胞(He La)增殖产生影响。方法:在培养的HeLa细胞,不同浓度(1,2,4μmol/L)连翘酯苷B处理24、48、72 h后采用MTT实验观察细胞活力变化。观察不同浓度的连翘酯苷B对肿瘤细胞克隆形成的影响。连翘酯苷B处理细胞后,观察转录因子NF-κB(p-p65、p65)蛋白调控变化,并检测蛋白p21、以及细胞周期蛋白Cyclin E、CDK2的表达变化。结果:连翘酯苷B浓度依赖性抑制肿瘤细胞克隆形成,且对HeLa细胞增殖效应的抑制呈时间及浓度依赖性。连翘酯苷B浓度依赖性上调p21蛋白水平,并且下调p-p65、Cyclin E与CDK2水平。结论:连翘酯苷B通过抑制转录因子NF-κB,影响p21/Cyclin E/CDK2信号通路抑制了HeLa细胞增殖。AIM:To study the effects of forsythiaside B on HeLa cells proliferation by inhibiting NF-κB through p21/Cyclin E/CDK2 signaling pathway.METHODS:The cell activity of HeLa cells was observed by MTT assay after 24 h,48 h and 72 h treatment with different concentration(1,2,4μmol/L)of forsythiaside B.The clone formation of HeLa cells was evaluated with the condition of different concentrations of forsythiaside B.The expression of transcription factor NF-κB(p-p65 and p65)was determined,and the expression of p21,Cyclin E and CDK2 were detected as well.RESULTS:Forsythioside B inhibited the clone formation of HeLa cells in concentration dependent manner,and the inhibitory effect on HeLa cells’proliferation by forsythioside B was in both time and concentration dependent manner.Forsythioside B up-regulated p21 protein expression and down-regulated p-p65,Cyclin E and CDK2 protein expression.CONCLUSION:Forsythioside B inhibits the transcription factor NF-κB and affects the p21/Cyclin E/CDK2 signaling pathway,thus inhibiting the proliferation of He La cells.

关 键 词：连翘酯苷B NF-ΚB HELA细胞 P21 

分 类 号：R737.33[医药卫生—肿瘤] R965.2[医药卫生—临床医学]