白内障术后房水OPN和PDGF-A水平检测与后发性白内障的相关性研究  

Relationship between the Levels of OPN and PDGF-A in Aqueous Humor and Secondary Cataract

作　　者：韩梅 苏晓明 杜建英 HAN Mei;SU Xiao-ming;DU Jian-ying(Department of Clinical Laboratory,the Hospital of Jingyang Country,Shaanxi Jingyang 713700,China;Department of Clinical Laboratory,the First People’s Hospital ofXianyang,Shaanxi Xianyang 712000,China;Department of Eye Qenter,the First People’s Hospital ofXianyang,Shaanxi Xianyang 712000,China)

机构地区：[1]陕西省咸阳市泾阳县医院检验科,陕西泾阳713700 [2]咸阳市第一人民医院检验科,陕西咸阳712000 [3]咸阳市第一人民医院眼科中心,陕西咸阳712000

出　　处：《现代检验医学杂志》2020年第2期46-48,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨白内障术后房水血小板衍生生长因子-A(platelet derived growth factor-A,PDGF-A)和骨桥蛋白(osteopontin,OPN)水平的变化与后发性白内障的相关性。方法收集2018年5月~2019年5月在咸阳市第一人民医院就诊的310例(310眼)白内障患者的临床资料进行回顾性分析。所有患者均行白内障超声乳化吸除加人工晶状体植入手术。依据患者术后晶状体后囊膜混浊(posterior capsule opacification,PCO)程度分为未混浊组(对照组186例),轻度混浊组(mild turbidity,MT,101例)和重度混浊组(severe turbidity,ST,23例)。通过视觉质量分析系统检测眼部客观散射指数(objective scattering index,OSI),采用酶联免疫吸附法检测PDGF-A和OPN水平。比较分析房水中PDGF-A和OPN水平的变化与后发性白内障的相关性。结果在对照组,MT组和ST组中,房水OPN(ng/ml),PDGF-A(ng/L)水平及OSI分别为509.13±69.26, 727.53±97.18和997.39±123.06;43.12±11.37, 72.39±15.90和113.67±23.45;0.62±0.21,1.57±0.42和6.29±1.51。与对照组比较,MT组和ST组的PDGF-A,OPN水平及OSI均显著增高,且ST组的PDGFA,OPN水平及OSI增高更显著(F=97.32~189.10,均P=0.000)。在MT及ST组中,房水PDGF-A和OPN水平具有正相关性(r=0.851,P<0.01),在MT及ST组中,房水PDGF-A和OPN水平分别与OSI有正相关性(rPDGF-A=0.813, 0.837,rOPN=0.803, 0.829,均P<0.01)。结论 PDGF-A和OPN与白内障术后的PCO有关,检测房水PDGF-A和OPN水平有助于预测后发性白内障的发生和发展。Objective To investigate the correlation between the changes of levels of platelet derived growth factor-A(PDGF-A) and osteopontin(OPN) in aqueous humor and the secondary cataract. Methods The clinical data of 310 cataract patients(310 eyes) treated in the First People’s Hospital of Xianyang City from May 2018 to May 2019 were collected for retrospective analysis.All patients underwent phacoemulsification and intraocular lens implantation. According to the degree of posterior capsular opacification, the patients were divided into three groups: non opacification group(control group, 186 cases), mild opacification group(MT, 101 cases) and severe opacification group(ST, 23 cases). Objective scattering index(OSI) was measured by vision quality analysis system. PDGF-A and OPN were detected by enzyme-linked immunosorbent assay. The correlation between PDGF-A and OPN levels in aqueous humor and posterior cataract was analyzed. Results In control group, MT group and ST Group, OPN(ng/ml),PDGF-A(ng/L) and OSI were 509.13±69.26, 727.53±97.18 and 997.39±123.06, 43.12±11.37, 72.39±15.90 and 113.67±23.45;0.62±0.21, 1.57±0.42 and 6.29±1.51, respectively. The levels of PDGF-A, OPN as well as OSI in MT group and ST group were significantly higher than those in control group, and the levels of PDGF-A, OPN as well as OSI in ST group more significantly increased(F=97.32~189.10, all P= 0.000). In MT and ST groups, there was a positive correlation between the levels of PDGF-A and OPN(r=0.851,P<0.01) and the MT and ST groups, there were positive correlations between the levels of PDGF-A and OPN, and OSI(rPDGF-A=0.813, 0.837, rOPN=0.803, 0.829, all P<0.01). Conclusion PDGF-A and OPN were related to posterior capsule opacification after cataract surgery. Detection of levels of PDGF-A and OPN in aqueous humor is helpful to predictt the occurrence and development of secondary cataract.

关 键 词：白内障术后 后发性白内障 后囊膜混浊 客观散射指数 血小板衍生生长因子-A 骨桥蛋白 

分 类 号：R776.1[医药卫生—眼科] R392.11[医药卫生—临床医学]