超声辅助提取泡桐叶熊果酸工艺研究  

Study on Ultrasonic-assisted Extraction of Ursolic Acid from Paulownia tomentosa Leaves

作　　者：陈静 董家新 赵丰丽[1] CHEN Jing;DONG Jia-xin;ZHAO Feng-li(Key Laboratory of Ecology of Rare and Endangered Species and Environmented Protection,Guangxi Normal University,Guilin,Guangxi 541004;School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin,Guangxi 541004)

机构地区：[1]广西师范大学,珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541004 [2]广西师范大学化学与药学学院,广西桂林541004

出　　处：《安徽农业科学》2020年第9期190-192,199,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(21463008);桂林市科学研究与技术开发计划项目(20171015-2)。

摘　　要：[目的]采用超声辅助提取泡桐叶中熊果酸,为泡桐熊果酸的开发利用提供基础。[方法]通过单因素试验和正交试验研究乙醇浓度、提取时间、提取温度、料液比、超声波功率和提取次数对熊果酸得率的影响。[结果]经方差分析和验证试验确定了从泡桐叶中提取熊果酸的最佳提取条件为乙醇浓度90%、料液比1:20(g:mL)、提取时间40 min、提取温度65℃、提取次数3次、超声波功率350 W,在该提取工艺条件下熊果酸得率为0.742%。[结论]超声法提取是一种效率高、条件温和且操作简单的大众化的提取熊果酸的方法。[Objective]In order to provide a basis for the development and utilization of ursolic acid from Paulownia tomentosa,ultrasonic assisted extraction was adopted to extract the ursolic acid from Paulownia tomentosa.[Method]The efects of ethanol concentration,extraction time,extraction temperature,solid-liquid ratio,ultrasonic power and extraction times on the yield of ursolic acid were studied by single factor and orthogonal experiments.[Result]The optimum extraction conditions of ursolic acid from Paulownia tomentosa leaves were determined by variance analysis and validation test,the concentration of ethanol was 90%,the solid-liquid ratio was 1:20,the extraction time was 40 min,the extraction temperature was 65℃,the extraction times was 3 times,and the ultrasonic power was 350 W.Under the condition of the extraction process,the ursolic acid yield was 0.742%.[Conclusion]Ultrasound extraction is a popular method to extract ursolic acid with high efficiency,mild conditions and simple operation.

关 键 词：泡桐叶 熊果酸 超声波辅助 提取 正交试验 

分 类 号：R284.2[医药卫生—中药学]