HNF3β对SW480细胞株增殖、侵袭和转移的影响  

Effects of HNF3βon cell proliferation,invasion and metastasis of SW480 cell line

作　　者：徐君毅[1] 宋学民[1] 以敏[2] XU Jun-yi;SONG Xue-min;YI Min(The Fourth Affiliated Hospital of Guangxi Medical University,Gastrointestinal Surgery,545005;The Fourth Affiliated Hospital of Guangxi Medical University,Pathology Department,545005)

机构地区：[1]广西医科大学第四附属医院胃肠外科,545005 [2]广西医科大学第四附属医院病理科,545005

出　　处：《现代消化及介入诊疗》2020年第4期467-471,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：广西自然科学基金(2014GXNSFBA118200);广西区卫生厅自筹经费课题(Z2011112)。

摘　　要：目的探究HNF3β对结直肠癌SW480细胞株细胞增殖、侵袭和转移的机制。方法体外培养结直肠癌SW480细胞株细胞,并分为对照组、HNF3β转染组和空转质粒组;构建包含HNF3β蛋白全长的peDNA3.1质粒对HNF3β转染组进行质粒转染,空转质粒组使用空白质粒转染;使用蛋白质印记检测各组细胞HNF3β蛋白表达情况;使用克隆形成实验检测细胞生长情况;使用Transwell小室检和划痕实验法测各组细胞运动能力;使用蛋白质印记检测增殖、侵袭转移相关蛋白质及JAK/STA信号通路相关蛋白表达情况。结果与对照组相比,HNF3β转染组HNF3β蛋白表达、E-cadherin蛋白表达显著升高(P<0.01),细胞克隆形成率、PCNA蛋白表达、Ki67蛋白表达、单位面积侵袭细胞数目、N-cadherin蛋白表达、Vimentin蛋白表达、JAK1蛋白表达、STAT3蛋白表达水平显著降低(P<0.01);相比HNF3β转染组,空转质粒组HNF3β蛋白表达、E-cadherin蛋白表达显著降低(P<0.01),细胞克隆形成率、PCNA蛋白表达、Ki67蛋白表达、单位面积侵袭细胞数目、N-cadherin蛋白表达、Vimentin蛋白表达、JAK1蛋白表达、STAT3蛋白表达水平显著升高(P<0.01)。结论HNF3β是结直肠癌的抑癌基因,可能通过调控JAK/STAT3信号通路抑制SW480细胞的增殖、侵袭和转移。Objective To explore effect mechanism of HNF3βon cell proliferation,invasion and metastasis of colorectal cancer SW480 cell line.Methods Colorectal cancer SW480 cell lines were cultured in vitro.They were divided into control group(Control),HNF3βtransfection group(HNF3β)and idle plasmid group(NC).The pe DNA3.1 plasmid containing full length of HNF3βprotein was constructed for plasmid transfection in HNF3βtransfection group.The blank plasmids were applied for transfection in idle plasmid group.The expression of HNF3βprotein in each group was detected by Western blot.The cells growth was detected by clone formation assay.The cell athletic ability in each group was detected by Transwell chamber test and scratch test.Western blot was applied to detect expression of proliferation,invasion and metastasis related proteins,and JAK/STA signaling pathway related proteins.Results Compared with control group,expression of HNF3βand E-cadherin proteins was significantly increased in HNF3βtransfection group(P<0.01),while cell clone formation rate,expression of PCNA and Ki67 proteins,number of invaded cells per unit area,expression levels of N-Cadherin,Vimentin,JAK1 and STAT3 proteins were significantly decreased(P<0.01).Compared with HNF3βtransfection group,expression of HNF3βand E-cadherin proteins was significantly decreased in idle plasmid group(P<0.01),while cell clone formation rate,expression of PCNA and Ki67 proteins,number of invaded cells per unit area,expression levels of N-Cadherin,Vimentin,JAK1 and STAT3 proteins were significantly increased(P<0.01).Conclusion HNF3βis a tumor suppressor gene for colorectal cancer,which may inhibit proliferation,invasion and metastasis of SW480 cells by regulating JAK/STAT3 signaling pathway.

关 键 词：结直肠癌 肝细胞核因子3β 细胞增殖 细胞侵袭 

分 类 号：R73-37[医药卫生—肿瘤] R574[医药卫生—临床医学]