白及多糖响应曲面法优化提取工艺及抑菌活性研究  

Study on the Optimized Extraction Process by Response Surface Methodology and their Antibacterial Activity of Polysaccharides from Bletilla Striata

作　　者：何晓梅[1,2,3] 申男 朱富成 乔德亮 张珍林[1,2,3] 杨静 HE Xiao-mei;SHEN Nan;ZHU Fu-cheng;QIAO De-liang;ZHANG Zhen-lin;YANG Jing(College of Biology and Pharmaceutical Engineering,West Anhui University,Liu’an 237012,China;Anhui Province Engineering Technology Research Center of Plant Cell Engineering,Lu’an 237012,China;Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources,Lu'an 237012,China)

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]植物细胞工程安徽省工程技术研究中心,安徽六安237012 [3]安徽省中药资源保护与持续利用工程实验室,安徽六安237012

出　　处：《宜春学院学报》2020年第3期13-16,36,共5页Journal of Yichun University

基　　金：安徽省教育厅重点项目(KJ2017A407,KJ2018A0420,KJ2016A887);安徽省级大学生创新项目(S201910376108);皖西学院校级重点项目(WXZR201622,WXZR201816)。

摘　　要：研究影响白及多糖提取的因素,优化其提取工艺及探究其抑菌活性。通过单因素和响应曲面法,选择提取次数、水提时间、水提温度、液料比4个因素筛选白及多糖的最佳水提工艺,蒽酮-硫酸法测定白及多糖的含量,滤纸片-抑菌圈法测定白及多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌及黑曲霉菌的抑菌作用。经由以上实验设计可得白及多糖最佳提取工艺为:提取次数1次、水提时间2.2 h、水提温度77℃、液料比39.27 mL/g,白及多糖得率31.09%;白及多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌及黑曲霉菌均有抑制作用。To study the factors affecting the extraction of Bletilla Striata polysaccharide,optimize the extraction process and explore its antibacterial activity.By single factor and response surface method,four factors including extraction times,water extraction time,water extraction temperature,liquid-material ratio were selected the best water extraction process for Bletilla Striata polysaccharide,anthrone-sulfuricacid method to determine the content ofBletilla Striata polysaccharide,bacteriostatic circle a method to determine Bletilla Striata polysaccharide in e.coli,staphylococcus aureus,bacillus subtilis and aspergillus bacteriostatic action.According to the above experimental design,the optimal extraction process of Bletilla Striata polysaccharide was as follows:extraction times 1,waterextraction temperature 77℃,water extraction time 2.2h,liquid-solid ratio 39.27mL/g,and yield of Bletilla Striata polysaccharide 31.09%.Bletilla Striata polysaccharide can inhibit Escherichia coli,Staphylococcus aureus,Bacillus subtilis andAspergillus Niger.

关 键 词：白及多糖 响应曲面法 提取工艺 抑菌活性 

分 类 号：TQ461[化学工程—制药化工]