MiR-646在前列腺癌及前列腺增生组织中的表达差异及临床意义  

The difference expression and its clinical value of MiR-646 in prostate cancer and prostatic hyperplasia

作　　者：陈勇杰 许春 CHEN Yong-jie;XU Chun(Department of Urology,Success hospital affiliated to Xiamen university,Xiamen,Fujian 361000,China)

机构地区：[1]厦门大学附属成功医院泌尿外科,福建厦门361000

出　　处：《实用医药杂志》2020年第5期401-403,408,共4页Practical Journal of Medicine & Pharmacy

摘　　要：目的探讨miRNA-646在前列腺癌及前列腺增生组织中的差异及临床意义。方法以2014年1月—2016年12月来院就诊的前列腺癌患者为试验组(78例),对照组为同期就诊的前列腺增生患者(78例)。应用RT-PCR方法检测前列腺癌组织及前列腺增生组织中的miRNA-646表达,分析miRNA-646在癌组织及良性前列腺组织中的表达水平的差异。对前列腺癌患者miR-646和血清TPSA水平进行相关性分析,同时分析在前列腺癌患者中miR-646的表达与临床特征关系。结果RT-PCR结果显示,试验组中前列腺癌组织中miR-646的表达水平显著低于前列腺增生组织,差异具有统计学意义(P<0.05);采用Sperman方法分析显示前列腺癌组织中miR-646和PSA的表达显示两者呈负相关(r=-0.267,P<0.001)。在前列腺癌患者中不同年龄、肿瘤大小患者中miR-646的表达差异无统计学意义(P>0.05);而不同Gleason分级、肿瘤分化程度及TNM分期患者miR-646的表达差异存在显著性(P=0.002;P=0.011;P=0.013),且Gleason分级、肿瘤恶性程度及TNM分期与miR-646的表达呈负相关(r=-0.368;r=-0.295;r=-0.336)。结论低表达miR-646提示了前列腺癌患者的预后不良,miR-646在评估前列腺癌的预后及早期诊断方面可能具有一定的临床应用价值。Objective To investigate the difference expression of miR-646 in prostate cancer and prostatic hyperplasia and its clinical significance.Methods Real-time reverse transcriptase polymerase chain reaction was used to detect miR-646 expression in 78 cases of prostate cancer and 78 cases of prostatic hyperplasia patients.The difference of miRNA-646 expression in cancer tissues and benign prostate tissues was analyzed.The correlation between miR-646 and serum TPSA levels in patients with prostate cancer and the relationship between the expression of miR-646 and clinical features were analyzed.Results The expression level of miR-646 was significantly lower in tumor tissues than that in prostatic hyperplasia tissue(P<0.05).The expression of miR-646 and PSA in prostate cancer tissues was negatively correlated by Sperman analysis(r=-0.267,P<0.001).Clinicopathological analysis showed that there was no significant relationship of miR-646 expression in 78 cases of prostate cancer tissues with age and tumor sizes(P>0.05),while they were negatively correlated with Gleason grade,umor differentiation degree and TNM staging of prostate cancer(r=-0.368;r=-0.295;r=-0.336).Conclusion The abnormal expression of miR-646 corelates with progression of prostate cancer,which can be maker of prostate cancer.Detection of miR-646 may be helpful for diagnosis and prognosis.

关 键 词：前列腺癌 miRNA-646 前列腺癌抗原 预后 

分 类 号：R737.2[医药卫生—肿瘤]