健胃消胀片对慢性萎缩性胃炎模型大鼠胃粘膜COX-2、P53和TFF2表达的影响  

Effects of jianwei xiaozhang tablet on the expression of COX-2,P53 and TFF2in gastric mucosa of rats with chronic atrophic gastritis

作　　者：陈诗园 董明国 CHEN Shi-yuan;DONG Ming-guo(Department of Medical Oncology,Dongguan Hospital of Traditional Chinese Medicine,Dongguan,Guangdong 523000;Department of Gastroenterology,Dongguan Hospital of Traditional Chinese Medicine,Dongguan,Guangdong 523000,China)

机构地区：[1]广州中医药大学附属东莞中医院肿瘤内科,广东东莞523000 [2]广州中医药大学附属东莞中医院脾胃科,广东东莞523000

出　　处：《热带医学杂志》2020年第2期173-175,207,F0002,共5页Journal of Tropical Medicine

基　　金：2017东莞市社会科技发展(重点)项目(201750715002434)。

摘　　要：目的研究健胃消胀片对慢性萎缩性胃炎(CAG)模型大鼠胃粘膜环氧合酶-2(COX-2)、P53蛋白和三叶因子-2(TFF2)表达的影响。方法采用脱氧胆酸钠、水杨酸钠交替灌胃并以甲基硝基亚硝基胍(MNNG)溶液作为饮用水造CAG模型,将大鼠随机分为正常组、模型组、对照组(胃复春)和健胃消胀片高、中、低剂量组。连续给药7周后采用HE染色观察胃组织病理学变化;并用免疫组化染色观察胃粘膜TFF2、COX-2和P53蛋白的表达。结果对照组和健胃消胀片组的动物死亡率均比模型组低,其中高剂量组的死亡率最低(23.5%),其次为低剂量组(25.0%)、中剂量组(35.3%)、对照组(35.3%)。免疫组化结果显示,模型组的COX-2、TFF2、P53光密度值均上升,与正常组比较差异有统计学意义(P<0.05);经7周治疗后,各治疗组COX-2、TFF2、P53蛋白均下降,与模型组比较差异有统计学意义(P<0.05)。结论动物研究显示健胃消胀片能够下调CAG大鼠模型胃粘膜TFF2、COX-2和P53蛋白的表达,增强粘膜屏障的防御和修复能力。Objective To analyze the effects of jianwei xiaozhang tablet on the expression of cyclooxygenase-2(COX-2),P53 and trefoil factor family-2(TFF2)in gastric mucosa of rats with chronic atrophic gastritis(CAG). Methods The CAG model was constructed by alternating gavage of sodium deoxycholate salicylate and using 1-MethyI-3-nitro-1-nitrosoguanidine(MNNG)as drinking water. The rats were randomly divided into the normal group,the model group,the positive control group(spring back)and the high-medium and low-dose jianwei xiaozhang tablet group,and the gastric histopathological changes were observed by HE staining after 7 weeks of continuous administration. The expression of TFF2,COX-2 and P53 proteins in gastric mucosa were observed by immunohistochemical staining. Results Animal mortality was lower in the treatment group than that in the model group. The lowest mortality was 23.5% in the high-dose group,followed by the lowdose group(25.0%),the medium-dose group(35.3%),and the control group(35.3%). Immunohistochemical results showed that the COX-2,TFF2 and P53 optical density values in the model group were increased,and the differences were extremely significant compared with those in the normal group(P<0.05). After 7 weeks of treatment,COX-2,TFF2 and P53 protein levels decreased in each treatment group,which was statistically significant compared with those in the model group(P<0.05). Conclusion Jianwei xiaozhang tablet could regulate the expression of gastric mucosa TFF2,COX-2 and P53 protein levels in CAG rat model,enhance the defense and repair ability of mucosal barrier,and thus might play a role in the treatment of CAG.

关 键 词：慢性萎缩性胃炎 健胃消胀片 三叶因子-2 环氧合酶-2 P53蛋白 

分 类 号：R573.32[医药卫生—消化系统]