广西人群miRNA-107基因多态性分布特征及其与血脂的关系  

Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels

作　　者：黄艳云 黄华佗 陆玉兰[1] 雷茗[1] 谭昙 韦叶生[1] HUANG Yan-yun;HUANG Hua-tuo;LU Yu-lan;LEI Ming;TAN Tan;WEI Ye-sheng(Department of Clinical Laboratory,Affiliated Hospital of Youjiang Medical University for Nationalities,Guangxi Baise 533000,China;Department of Clinical Laboratory,People's Hospital of Baise,Guangxi Baise 533000,China)

机构地区：[1]右江民族医学院附属医院检验科,广西百色533000 [2]百色市人民医院检验科,广西百色533000

出　　处：《解剖学报》2020年第2期284-288,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(81560552);广西自然科学基金(2018GXNSFAA138120)。

摘　　要：目的探讨miR-107基因单核苷酸多态性(SNP)位点rs2296616 C/T在广西地区健康人群中的分布特点,对比其在不同种族间基因型及等位基因频率分布的差异,并进一步探讨rs2296616 C/T位点单核苷酸多态性(SNP)与血脂水平的相关性。方法采用多重单碱基延伸SNP分型技术(multiplex SNa Pshot)和DNA测序法,检测372例广西健康人rs2296616 C/T位点的多态性,用7600生化仪检测其血脂相关指标,并用统计学方法分别比较rs2296616C/T位点多态性在各种族人群间的分布差异及不同基因型间的血脂水平差异。结果广西人群miR-107基因rs2296616 C/T位点存在TT(91.1%)和CT(8.9%)两种基因型及T(95.6%)和C(4.4%)两种等位基因。该位点的基因型和等位基因型频率在广西人群不同性别间的比较,差异无统计学意义(P>0.05)。其基因型和等位基因频率与人类基因组单体型图(Hap Map)所公布的欧洲人、日本人、非洲人、印第安人和墨西哥人分型数据相比较,差异均有统计学意义(P<0.05),但与北京汉族人群比较,差异无统计学意义(P>0.05)。rs2296616 C/T位点两种基因型人群血脂之间比较,携带TT基因型人群的高密度脂蛋白胆固醇(HDL-C)与CT组比较,差异具有统计学意义(P<0.05)。结论广西人群miR-107基因rs2296616 C/T位点多态性与其他种族人群之间比较存在不同程度的差异;rs2296616 C/T位点多态性与HDL-C水平高低有关。Objective To study the distribution characteristics of single nucleotide polymorphisms(SNP)of miR-107 gene rs2296616 C/T in Guangxi healthy population and comparison with that in different ethnic populations,and further to explore the correlation between rs2296616 C/T SNP and blood lipid level.Methods The polymorphisms of miR-107 gene rs2296616 C/T among 372 Chinese healthy individuals of Guangxi were detected by multiplex SNaPshot and DNA sequencing method,and the blood lipid-related indexes were detected by 7600 biochemical analyzer.The distribution of rs2296616 C/T polymorphism among different ethnic groups and the differences of blood lipid levels among different genotypes were compared by statistical method.Results MiR-107 gene rs2296616 C/T SNP contained TT(91.1%),CT(8.9%)genotypes and T(95.6%),C(4.4%)alleles in Guangxi healthy population.The frequencies of genotype and allele distribution of rs2296616 C/T were not significantly different among genders in Guangxi population(P>0.05).However,there were significant differences in the genotype and allele frequency of miR-107 gene rs2296616 C/T in Guangxi healthy population compared with those of Europeans,Japanese,Africans,Mexicans and Indians published in Hap Map(P<0.05),no significant difference compared with Hap Map-HGB(P>0.05).When compared the blood lipid level among two genotypes in rs2296616 C/T,we found that the level of high density lipoprotein cholesterol(HDL-C)with TT genotype was significantly different from that of CT group(P<0.05).Conclusion There are different degrees of variation in the polymorphisms of rs2296616 C/T of miR-107 gene between Guangxi people and other ethnic populations.The polymorphism of rs2296616 C/T locus is related to the level of HDL-C.

关 键 词：miR-107基因 单核苷酸多态性 rs2296616 C/T位点 种族 血脂 多重单碱基延伸SNP分型技术 DNA测序法 广西人群 

分 类 号：R394.3[医药卫生—医学遗传学]