5-ALA-PDT诱导人结肠癌HT-29细胞凋亡的机制研究  

Effect and apoptosis mechanism of human colonic carcinoma HT-29 cells induced by 5-ALA-PDT

作　　者：王韬[1] 聂双发[1] 薛军[1] 史浩天 王成瑶 胡晓峰[1] 费建东[1] 安永铸[1] Wang Tao;Nie Shuangfa;Xue Jun;Shi Haotian;Wang Chengyao;Hu Xiaofeng;Fei Jiandong;An Yongzhu(Department of General Surgery,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China;Blood Bank,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China;Image Center,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院普外科,张家口075000 [2]河北北方学院附属第一医院血库,张家口075000 [3]河北北方学院附属第一医院影像中心,张家口075000

出　　处：《中国医师杂志》2020年第4期486-489,494,共5页Journal of Chinese Physician

基　　金：河北省卫计委2018年度医学科学研究重点课题计划(20180821)。

摘　　要：目的探讨5-氨基乙酰丙酸(5-ALA)通过光动力疗法(PDT)诱导人结肠癌HT-29细胞凋亡的影响及其机制。方法将人结肠癌HT-29细胞分成4组:空白对照组、5-ALA组、PDT组、5-ALA-PDT组。对照组不给予光敏剂和照光处理,5-ALA组仅给予光敏剂不给予照光处理,PDT组仅给予照光处理不给予光敏剂,5-ALA-PDT组同时给予光敏剂和照光处理。流式细胞术观察HT-29细胞的凋亡情况,逆转录聚合酶链反应(RT-PCR)观察HT-29细胞B型淋巴细胞瘤-2(Bcl-2)及Bcl-2相关X蛋白(Bax)表达情况,利用紫外分光光度法检测含半胱氨酸的天冬氨酸水解酶-3(Caspase-3)表达量的变化。结果5-ALA-PDT组凋亡率与空白对照组、5-ALA组、PDT组比较,差异均有统计学意义(P<0.05)。5-ALA-PDT组Bcl-2表达与空白对照组、5-ALA组、PDT组比较,差异均有统计学意义(P<0.05);而4组间Bax表达比较,差异均无统计学意义(P>0.05)。5-ALA-PDT组Bax/Bcl-2表达与空白对照组、5-ALA组、PDT组比较,差异均有统计学意义(P<0.05)。5-ALA-PDT组Caspase-3表达与空白对照组、5-ALA组、PDT组比较,差异均有统计学意义(P<0.05)。结论5-ALA-PDT可诱导HT-29细胞凋亡,其机制可能与通过Bax/Bcl-2途径诱导细胞凋亡有关。Objective To explore the effect and mechanism of 5-Aminolevulinic Acid-Photodynamic Therapy(5-ALA-PDT)on the apoptosis of the human colonic carcinoma HT-29 cells.Methods HT-29 cells were cultured in vivio and divided into four groups:blank control group,5-ALA group,PDT group and 5-ALA-PDT group.The control group was not given photosensitizer and light treatment;5-ALA group was given photosensitizer;PDT group was given light treatment;5-ALA-PDT group was given photosensitizer and light treatment at the same time.Flow cytometry was used to observe the apoptosis of HT-29 cells.Reverse transcription polymerase chain reaction(RT-PCR)was used to observe the expression of B-type lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax)in HT-29 cells.Ultraviolet spectrophotometry was used to detect the expression of Caspase-3.Results The apoptotic rate of 5-ALA-PDT group was significantly higher than that of blank control group,5-ALA group and PDT group(P<0.05).Compared with the blank control group,5-ALA-PDT group and PDT group,the expression of Bcl-2 in the 5-ALA-PDT group was statistically significant(P<0.05),but there was no significant difference in Bax expression among the four groups(P>0.05).The expression of Bax/Bcl-2 in 5-ALA-PDT group was significantly higher than that in blank control group,5-ALA group and PDT group(P<0.05).The expression of Caspase-3 in 5-ALA-PDT group was significantly higher than that in blank control group,5-ALA group and PDT group(P<0.05).Conclusions 5-ALA-PDT can induce apoptosis of HT-29 cells,and its mechanism may be related to the induction of apoptosis through Bax/Bcl-2 pathway.

关 键 词：乙酰丙酸类 光化学疗法 HT29细胞 细胞凋亡 BCL-2相关X蛋白质 BCL-2蛋白 

分 类 号：R735.35[医药卫生—肿瘤]