Evaluation of oxidative stress levels in obesity and diabetes by the free oxygen radical test and free oxygen radical defence assays and correlations with anthropometric and laboratory parameters  

作　　者：Mihnea-Alexandru Găman Mirela Elena Epîngeac Camelia Cristina Diaconu Amelia Maria Găman 

机构地区：[1]"Carol Davila"University of Medicine and Pharmacy,Bucharest 050474,Romania [2]Department of Hematology,Center of Hematology and Bone Marrow Transplantation,Fundeni Clinical Institute,Bucharest 022328,Romania [3]Department of Pathophysiology,University of Medicine and Pharmacy of Craiova,Craiova 200349,Romania [4]Internal Medicine Clinic,Clinical Emergency Hospital of Bucharest,Bucharest 014461,Romania [5]Clinic of Hematology,Filantropia City Hospital,Craiova 200143,Romania

出　　处：《World Journal of Diabetes》2020年第5期193-201,共9页世界糖尿病杂志（英文版）（电子版）

摘　　要：BACKGROUND Obesity and diabetes are associated with high levels of oxidative stress.In Romanian patients with obesity and(or)diabetes,this association has not been sufficiently explored.AIM To evaluate oxidative stress in obese and(or)diabetic subjects and to investigate the possible correlations between oxidative stress and anthropometric/biochemical parameters.METHODS Oxidative stress was evaluated from a single drop of capillary blood.Reactive oxygen species(ROS)were evaluated using the free oxygen radical test(FORT).The free oxygen radical defence(FORD)assay was used to measure antioxidant levels.RESULTS FORT levels were higher in obese subjects(3.04±0.36 mmol/L H2O2)vs controls(2.03±0.14 mmol/L H2O2)(P<0.0001).FORD levels were lower in obese subjects(1.27±0.13 mmol/L Trolox)vs controls(1.87±1.20 mmol/L Trolox)(P=0.0072).Obese diabetic subjects had higher FORT values(3.16±0.39 mmol/L H2O2)vs non-diabetic counterparts(2.99±0.33 mmol/L H2O2)(P=0.0233).In obese subjects,FORT values correlated positively with body mass index(BMI)(r=0.48,P=0.0000),waist circumference(WC)(r=0.31,P=0.0018),fasting plasma glucose(FPG)(r=0.31,P=0.0017),total cholesterol(TC)(r=0.27,P=0.0068)and uric acid(r=0.36,P=0.0001).FORD values correlated negatively with BMI(r=-0.43,P=0.00001),WC(r=-0.28,P=0.0049),FPG(r=-0.25,P=0.0130),TC(r=-0.23,P=0.0198)and uric acid(r=-0.35,P=0.0002).In obese diabetic subjects,FORT values correlated positively with BMI(r=0.49,P=0.0034)and TC(r=0.54,P=0.0217).FORD values were negatively associated with BMI(r=-0.54,P=0.0217)and TC(r=-0.58,P=0.0121).CONCLUSION Oxidative stress levels,as measured by the FORT and FORD assays,were higher in obese subjects vs controls.ROS levels were elevated in diabetic obese patients vs obese non-diabetic patients and controls.

关 键 词：Oxidative stress OBESITY DIABETES Reactive oxygen species ANTIOXIDANTS DYSLIPIDAEMIA 

分 类 号：R58[医药卫生—内分泌]