纤维素可及性对木质纤维酶水解影响的研究进展  

Progress of the Effects of Cellulose Accessibility on Enzymatic Hydrolysis of Lignocellulose

作　　者：王博伟 闫雪晴 何贤 邹春阳 吴文娟[1] WANG Bo-wei;YAN Xue-qing;ZOU Chun-yang;HE Xian;WU Wen-juan(Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037

出　　处：《纤维素科学与技术》2020年第1期61-68,共8页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金青年基金(21704045);南京林业大学大学生创新训练计划项目(2018NFUSPITP673);江苏省高等学校大学生创新创业训练计划项目(201910298034Y)。

摘　　要：纤维素的可及性是纤维原料中与纤维素酶结合且能够被酶处理的结合位点量,与纤维原料中的粒径和孔隙度有关,纤维素的可及性是影响纤维素酶水解的关键因素。综述了当前纤维素可及性的测定方法,包括氮吸附法、水银压入法、溶质排斥法、蛋白质吸附法、Simons法等,总结了通过提高纤维素可及性来实现高效生物转化的预处理技术,并给出当前生物乙醇转化的可能研究方向。Lignocellulosic materials are among the most promising renewable energy and clean biomass resources in nature that can be utilized to produce fuel ethanol.There are many factors that affect the conversion of lignocellulosic materials to fuel ethanol,among which cellulose accessibility,the number of binding sites could be bound and actioned to the cellulase,has been proposed as a key factor in the efficient enzymatic hydrolysis of lignocellulosic biomass.In this article,an overview of the current pretreatment technologies to realize efficient biotransformation through increasing cellulose accessibility as well as most commonly used determination method of assessing cellulose accessibility,including nitrogen adsorption,mercury porosimetry,solute exclusion,protein adsorption and Simons’stain are presented.the possible research direction of the current bioethanol conversion based on lignocellulosic biomass is discussed.

关 键 词：纤维素 可及性 木质纤维酶 酶水解 木质纤维原料 

分 类 号：TQ352.6[化学工程] O636.2[理学—高分子化学]