核酸杂交介导的荧光信号放大方法在生物检测中的应用研究进展  

A Review on Application of Nucleic Acid Hybridization-Mediated Fluorescence Signal Amplification in Biological Detection

作　　者：许恒毅[1] 余双[1] 李晖 朱新根 张晶晶 XU Hengyi;YU Shuang;LI Hui;ZHU Xingen;ZHANG Jingjing(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)

机构地区：[1]食品科学与技术国家重点实验室,南昌大学,江西南昌330047

出　　处：《食品与生物技术学报》2020年第4期1-8,共8页Journal of Food Science and Biotechnology

基　　金：食品科学与技术国家重点实验室自由探索课题(SKLF-ZZB-201720);江西省教育厅基金项目(GJJ150014)。

摘　　要：核酸杂交介导的荧光信号放大方法是指通过目标物触发或阻断核酸杂交反应,引起核酸杂交产物上标记的荧光信号分子数目发生变化,从而实现荧光信号放大的一类方法。该方法因具有设计灵活,特异性好,灵敏度高,响应时间短以及背景信号低等优点,现已广泛用于生物检测。作者介绍了该方法在细菌、核酸、蛋白质、生物小分子和无机金属离子检测中的研究近况,并对其存在的问题和发展趋势进行了阐述和展望。Nucleic acids hybridization mediated fluorescence signal amplification methods refer to a kind of methods of triggering or blocking the hybridization of nucleic acids by targets,thus causing changes in the number of labeled fluorescence on the hybridization products.Because of flexible design,good specificity,high sensitivity,short response time and low background signal,these methods have been widely used in biological detection.This article reviewed the applications of these methods in the detection of bacteria,nucleic acids,proteins,biological small molecules and inorganic metal ions,as well as their problems and development tendency.

关 键 词：核酸 荧光 放大 检测 

分 类 号：TS201.6[轻工技术与工程—食品科学]