M-CSF诱导人骨髓间充质干细胞分化为肝样细胞的分子机制  

Molecular Mechanism of Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Hepatoid Cells Induced by M-CSF

作　　者：李伟[1] 陶晖[1] 曹云[1] 高松森 杨庆国[1] Li Wei;Tao Hui;Cao Yun;Gao Songsen;Yang Qingguo(The Ist Affiliated Hospital of Anhui Medical University,Hefei,230000)

机构地区：[1]安徽医科大学第一附属医院,合肥230000

出　　处：《基因组学与应用生物学》2020年第2期732-736,共5页Genomics and Applied Biology

摘　　要：本研究主要目的是明确M-CSF诱导骨髓间充质干细胞分化为肝样细胞的分子机制,为临床中的肝移植和治疗肝病提供新思路。对取自于本院骨科治疗的患者的股骨骨髓间充质干细胞进行提取、分离、传代培养及鉴定。流式细胞仪检测BMSCs的表面表型。为了诱导BMSCs的肝分化,本研究将BMSCs加入到培养基中。骨髓间充质干细胞诱导21 d后,BMSCs表达了肝细胞特异性标志物a-蛋白(AFP)和细胞角蛋白18(CK18),通过免疫荧光染色证实了分化与为分化的BMSCs表达的差异性。分化的BMSCs还显示了肝细胞的体外功能特征,包括白蛋白产生、尿素分泌和糖原储存。本研究结果表明,BMSCs在M-CSF诱导下可分化为功能性肝细胞样细胞,可作为肝病治疗的细胞来源。The main purpose of this study is to clarify the molecular mechanism of M-CSF inducing bone marrow mesenchymal stem cells to differentiate into hepatocyte-like cells,and to provide new ideas for clinical liver transplantation and treatment of liver diseases.The bone marrow mesenchymal stem cells from femur of our hospital were extracted,isolated,cultured and identified.The surface phenotype of BMSCs was detected by flow cytometry.In order to induce liver differentiation of BMSCs,we added BMSCs into the culture medium.BMSCs expressed hepatocyte-specific markers A-protein(AFP)and cytokeratin 18(CK18),21 days after induction of bone marrow mesenchymal stem cells.The difference between differentiated and differentiated BMSCs was confirmed by immunofluorescence staining.Differentiated BMSCs also showed in vitro functional characteristics of hepatocytes,including albumin production,urea secretion and glycogen storage.In conclusion,BMSCs can differentiate into functional hepatocyte-like cells induced by M-CSF,which can be used as a cell source for the treatment of liver diseases.

关 键 词：M-CSF BMSCS 骨髓间充质干细胞 细胞角蛋白 

分 类 号：R575[医药卫生—消化系统]