景衣安神散穴位贴敷对失眠大鼠神经递质影响的实验研究  

Experimental study on the effect of acupoint application of Jingyi Anshen Powder on neurotransmitters in rats with insomnia

作　　者：胡金霞 卢军 王洪霞 江钰 刘涛 胡晓灵 HU Jinxia;LU Jun;WANG Hongxia;JIANG Yu;LIU Tao;HU Xiaoling(Department of Geriatrics,Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine,Xinjiang Uygur Autonomous Region,Urumqi830000,China;Department of Pharmacy,Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine,Xinjiang Uygur Autonomous Region,Urumqi830000,China)

机构地区：[1]新疆维吾尔自治区中医药研究院老年病科,新疆乌鲁木齐830000 [2]新疆维吾尔自治区中医药研究院药学部,新疆乌鲁木齐830000

出　　处：《中国医药导报》2020年第10期13-17,共5页China Medical Herald

基　　金：新疆维吾尔自治区自然科学基金项目(2017D01 C160)。

摘　　要：目的探讨景衣安神散穴位贴敷对失眠大鼠神经递质的影响。方法将雄性大鼠64只按随机数字表法分为四组,每组16只,分别为对照组、模型组、空白组、实验组。对照组腹腔注射0.9%氯化钠溶液(1 mL/100 g),1次/d,连续注射2 d,不进行任何干预,连续饲养7 d。模型组、空白组、实验组均采用腹腔注射对苯丙氨酸(PCPA),1次/d,连续注射2 d,制造睡眠剥夺模型。模型组造模后,不进行任何干预,连续饲养7 d。空白组造模后,将空白敷膜贴于神阙、内关穴,1次/d,30 min/次,连续7 d。实验组造模后,将景衣安神散贴敷于神阙、内关穴,1次/d,30 min/次,连续7 d。实验第8天,大鼠断头,开颅取出脑组织,分离出下丘脑,洗净剪碎,匀浆取上清液,采用酶联免疫吸附测定法检测5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、多巴胺(DA)、去甲肾上腺素(NANE)含量。结果模型组、空白组、实验组大鼠的力竭时间均短于对照组,绝望时间均长于对照组,差异有高度统计学意义(P <0.01)。空白组、实验组大鼠的力竭时间均长于模型组,绝望时间均短于模型组,差异有高度统计学意义(P <0.01)。模型组、空白组大鼠下丘脑5-HT含量均低于对照组,实验组大鼠下丘脑5-HT含量高于模型组、空白组,差异有统计学意义(P <0.05或P <0.01)。模型组、空白组大鼠下丘脑5-HIAA含量均低于对照组,实验组大鼠下丘脑5-HIAA含量高于模型组,差异有高度统计学意义(P <0.01)。模型组、空白组大鼠下丘脑DA含量均高于对照组,实验组大鼠下丘脑DA含量低于模型组,差异有高度统计学意义(P <0.01)。模型组、空白组大鼠下丘脑NANE含量均高于对照组,实验组大鼠下丘脑NANE含量低于模型组、空白组,差异有统计学意义(P <0.05或P <0.01)。结论景衣安神散穴位贴敷失眠大鼠可能通过影响下丘脑神经递质水平的变化来改善睡眠。Objective To explore the effect of acupoint application of Jingyi Anshen Powder on neurotransmitters in rats with insomnia.Methods According to the random number table method,64 male rats were divided into four groups:control group(n=16),model group(n=16),blank group(n=16)and experimental group(n=16).Control group was intraperitoneally injected with 0.9%Sodium Chloride Solution(1 mL/100 g)once a day for 2 days,and fed continuously for 7 days without any intervention.Model group,blank group and experimental group were all injectedintraperitoneally with P-phenylalanine(PCPA)once a day for two consecutive days to create a sleep deprivation model.After molding,model group was fed for7 days without any intervention.After molding,in blank group,blank film was applied to shenque and neiguan acupoints once a day,30 minutes a time for 7 days.After molding,in experimental group,Jingyi Anshen Powder was applied to shénquē and ■acupoints once a day for 30 minutes for 7 days.On the 8th day of the experiment,the rats were decapitated,the brain tissues were taken out after craniotomy,the hypothalamus was separated,washed and chopped,and the supernatant was taken from the homogenate.Contents of 5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA),dopamine(DA)and noradrenaline(NANE)were measured by enzyme-linked immunosorbent assay.Results The exhaustion time of model group,blank group and experimental group was shorter than that of control group,and the despair time of model group,blank group and experimental group was longer than that of control group,the differences were statistically significant(P<0.01).The exhaustion time of blank group and experimental group was longer than that of model group,and the desperation time of blank group and experimental group was shorter than that of model group,the differences were highly statistically significant(P<0.01).The contents of 5-HT in model group and blank group were lower than that in control group,the contents of 5-HT in experimental group was higher than that in model

关 键 词：景衣安神散 穴位贴敷 动物实验 单胺递质 

分 类 号：R-332[医药卫生]