胎盘间充质干细胞对大鼠子宫内膜损伤的修复作用  

Repair effect of placental mesenchymal stem cells on endometrial injury in rats

作　　者：王香香 王焱[1,2] 杨稍稍 张俊俊 WANG Xiangxiang;WANG Yan;YANG Shaoshao;ZHANG Junjun(College of Clinical Medical,He′nan University of Science and Technology,He′nan Province,Luoyang 471003,China;Department of Obstetrics and Gynecology,the First Affiliated Hospital of He′nan University of Science and Technology,He′nan Province,Luoyang 471003,China)

机构地区：[1]河南科技大学临床医学院,河南洛阳471003 [2]河南科技大学第一附属医院妇产科,河南洛阳471003

出　　处：《中国医药导报》2020年第10期18-21,41,F0003,共6页China Medical Herald

摘　　要：目的研究胎盘间充质干细胞对大鼠子宫内膜损伤的修复作用。方法 2019年5~11月使用化学损伤法对60只雌性SD大鼠(南京市江宁区青龙山动物繁殖场)构建子宫内膜损伤模型,采用单纯随机抽样分为对照组、凝胶组、模型组,每组各20只。3个动情周期后,每组采用单纯随机抽样抽取10只大鼠行HE染色测量子宫内膜腺体数目、子宫内膜厚度,Masson染色测量子宫内膜纤维化面积百分比。其他30只大鼠与雄鼠合笼,受孕第10天,开腹观察大鼠子宫胚胎个数。结果与对照组比较,模型组子宫内膜腺体数目减少、子宫内膜厚度变薄、子宫内膜纤维化面积百分比升高、受孕大鼠子宫胚胎个数减少,差异有统计学意义(P <0.05)。与模型组比较,凝胶组子宫内膜腺体数目增加、子宫内膜厚度增高、子宫内膜纤维化面积百分比降低,受孕大鼠子宫胚胎个数增多,差异有统计学意义(P <0.05)。与对照组比较,凝胶组子宫内膜腺体数目、子宫内膜厚度无明显变化,差异无统计学意义(P> 0.05);子宫内膜纤维化面积百分比升高,受孕大鼠子宫胚胎个数减少,差异有统计学意义(P <0.05)。结论胎盘间充质干细胞可一定程度地修复损伤的子宫内膜,增加子宫内膜腺体数目、子宫内膜厚度,降低子宫内膜纤维化面积百分比,提高胚胎着床率。Objective To study the repair effect of placental mesenchymal stem cells on endometrial injury in rats.Methods From May to November 2019, a total of 60 female SD rats(Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing) were constructed endometrial injury models by chemical damage method, and they were divided into control group, gel group and model group by simple random sampling, with 20 rats in each group. After three estrous cycles, 10 rats from each group were randomly sampled for HE staining, and the number of endometrial glands and endometrial thickness were measured, while the percentage of endometrial fibrosis were measured by Masson staining. The other 30 rats were caged with male rats. On the 10 th day of conception, the number of uterine embryos in the rats was observed by laparotomy. Results Compared with the control group, the number of endometrial glands decreased, the thickness of the endometrium became thinner, the percentage of endometrial fibrosis increased, while the number of uterine embryos in pregnant rats decreased in the model group, and the differences were statistically significant(P <0.05). Compared with the model group, the number of endometrial glands increased, the thickness of the endometrium increased, the percentage of endometrial fibrosis area decreased, while the number of uterine embryos in pregnant rats increased in the gel group, and the differences were statistically significant(P < 0.05). Compared with the control group,the number of endometrial glands and endometrial thickness had no significant changes in the gel group, and the differences were not statistically significant(P > 0.05);while the percentage of endometrial fibrosis increased, the number of uterine embryos in pregnant rats decreased, and the differences were statistically significant(P < 0.05). Conclusion Placental mesenchymal stem cells can repair damaged endometrium, increase the number of endometrial glands and endometrial thickness, reduce the percentage of endometrial fibrosis area, and improv

关 键 词：胎盘间充质干细胞 子宫内膜损伤 子宫内膜腺体计数 子宫内膜厚度 子宫内膜纤维化面积百分比 

分 类 号：R730.53[医药卫生—肿瘤]