结直肠腺癌中浸润性免疫细胞占比与预后的关系  

The proportion and prognostic correlation of infiltrating immune cells in colorectal adenocarcinoma

作　　者：李嵘祺 曹亚文 沈粤春[2] 李君[1] Li Rongqi;Cao Yawen;Shen Yuechun;Li Jun(Department of Hepatobiliary Surgery,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China;Department of Cardiology,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China)

机构地区：[1]广州医科大学附属第一医院肝胆外科,510120 [2]广州医科大学附属第一医院心内科,510120

出　　处：《中华普通外科杂志》2020年第4期284-287,共4页Chinese Journal of General Surgery

基　　金：广州市科技计划(201804010021)。

摘　　要：目的研究结直肠腺癌中肿瘤浸润性免疫细胞(TIICs)的相对占比,探讨TIICs与结直肠腺癌的预后及临床分期的关系。方法通过TCGA数据库获取结直肠腺癌基因转录表达数据及样本对应的临床信息。通过CIBERSORT软件计算每个样本中22种TIICs的相对占比。利用R软件分析结直肠腺癌和正常结肠组织之间TIICs的占比差异;分别对每种TIICs做单因素生存分析,并分析每种TIICs与结直肠腺癌临床分期之间的关系。结果从TCGA数据库中一共得到514例样本的基因转录表达数据和临床信息,其中结直肠腺癌473例,正常癌旁组织41例。发现幼稚B细胞等12种TIICs在结直肠腺癌和正常结直肠组织中差异均有统计学意义(均P<0.05)。经过样本临床信息的对应配对后,纳入到生存分析共222例。对每种TIICs相对比例值降序排列,按中位值将所有样本分为高、低浸润组,单因素生存分析结果发现,记忆B细胞对结直肠腺癌预后的影响有统计学意义(P<0.05)。活化CD4记忆T细胞等4种TIICs在结直肠腺癌的不同临床分期中占比差异均有统计学意义(均P<0.05)。结论TIICs与结直肠腺癌的预后及临床分期有关。Objective To study the relative proportion of tumorinfiltrating immune cells(TIICs)in colorectal adenocarcinoma(CRC),and to explore the correlation between TIICs and CRC in prognosis and clinical staging.Methods CRC gene transcriptional expression data and clinical information were obtained from TCGA database.The CIBERSORT software was used to calculate the relative proportions of 22 TIICs in each sample.R software was used to compare the proportion of TIICs between CRC and normal tissues.Single factor survival analysis was performed for each TIICs.Finally,the correlation between each TIICs and CRC clinical stage was studied.Results A total of 514 gene transcriptional expression data and clinical information were obtained from TCGA database,including 473 CRC and 41 normal adjacent tissues.The relative proportions of 22 TIICs in each sample were calculated using the CIBERSORT software u deconvolution methodn.In the study,12 TIICs including naive B cells were found to have statistically significant differences between CRC and normal tissues(all P<0.05).After matching the clinical information of the samples,a total of 222 cases were included in the survival analysis.The relative proportion of each TIICs was arranged in descending order,and all samples were divided into high and low infiltration groups according to the median value.Then,univariate survival analysis was performed for each TIICs,and it was found that memory B cells had a statistically significant effect on the prognosis of CRC(P<0.05).It was found that the proportion of four types of TIICs,including activated CD4 memory T cells,in different CRC clinical staging was statistically differe(all P<0.05).Conclusion TIICs is related to the prognosis and clinical stage of CRC.

关 键 词：结直肠肿瘤 免疫 细胞 预后 

分 类 号：R735.34[医药卫生—肿瘤]