Delphinidin-induced autophagy protects pancreatic β cells against apoptosis resulting from high-glucose stress via AMPK signaling pathway  

作　　者：Dengni Lai Mingyong Huang Lingyan Zhao Yan Tian Yong Li Dongpo Liu Yanyang Wu Fangming Deng 

机构地区：[1]Key Laboratory for Food Science and Biotechnology of Hunan Province,College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China [2]Iianxiang Biotechnology Co.,Ltd of Hunan,Shaoyang 422000,China [3]State Key Laboratory of Subhealth Intervention Technology,Changsha 410128,China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第12期1242-1249,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Industry Technical System for Specialized Vegetable(No.NCARS-24-&02)and the'1515'Talent Cultivation Plan of Hunan Agricultural University.

摘　　要：Hyperglycemia,a diagnostic characteristic of diabetes mellitus,is detrimental to pancreatic 0 cells.Delphinidin,a member of the anthocyanin family,inhibits glucose absorption,increases glucagonlike peptide(GLP-1)secretion,and improves insulin secretion in diabetes.However,whether delphinidin plays a protective role in pancreatic p-cell mass and function is not clear.In this study,delphinidin was found to decrease the high-glucose-induced apoptosis of RIN-m5F pancreatic β cells.In addition,delphinidin induced autophagy in RIN-m5F cells under the normal and highglucose conditions,while 3-methyladenine(3-MA)inhibition of autophagy significantly diminished the protective role of delphinidin against high-glucose-induced apoptosis of pancreatic β cells.Delphinidin also decreased the level of cleaved caspase 3 and increased the phosphorylation level of AMP-activated protein kinase a(AMPKa)Thr172.Compound C,an AMPK inhibitor,was found to decrease the ratio of LC3-II/LC3-I,and the apoptotic rate of high-glucose-injured cells was in creased after treatme nt with delphinidin,in dicating that delphinidi n attenuated the n egative effects of high-glucose stress to cells.In con elusion,our data dem on strate that delphi nidin protects pancreatic B cells against high-glucose-induced injury by autophagy regulation via the AMPK signaling pathway.These findings might shed light on the underlying mechanisms of diabetes and help improve the prevention and therapy of this common disease.

关 键 词：DELPHINIDIN high GLUCOSE AUTOPHAGY PANCREATIC B cells APOPTOSIS 

分 类 号：R58[医药卫生—内分泌]