前列腺癌中微小RNA-362-3p的表达及意义  

Expression and significance of microRNA-362-3p in prostate adenocarcinoma

作　　者：王刚[1] 姚丽霞[2] 杨涛[1] 彭克楠 郭留雄[1] 魏东[1] 谷守义[1] 刘俊江[1] WANG Gang;YAO Lixia;YANG Tao;PENG Kenang;GUO Liuxiong;WEI Dong;GU Shouyi;LIU Junjiang(Department of Urology,Hebei People s Hospital,Shijiazhuang 050051,Hebei,China;Department of Geriatrics,Hebei People s Hospital,Shijiazhuang 050051,Hebei,China;Clinical Laboratory,Hebei People s Hospital,Shijiazhuang 050051,Hebei,China)

机构地区：[1]河北省人民医院泌尿外科,河北石家庄050051 [2]河北省人民医院老年病二科,河北石家庄050051 [3]河北省人民医院检验科,河北石家庄050051

出　　处：《中南医学科学杂志》2020年第3期279-283,共5页Medical Science Journal of Central South China

基　　金：2018年度河北省医学科学研究重点课题计划(20180181);2019年度河北省医学科学研究课题计划(20190287).

摘　　要：为探索前列腺癌中微小RNA-362-3p(miR-362-3p)与转移的关系,以前列腺癌组织作为观察组,前列腺增生组织作为对照组,每组67例。采用实时荧光定量PCR法检测miR-362-3p的表达,免疫组化法检测上皮型粘附素(E-cadherin)和基质金属蛋白酶-11(MMP-11)的表达,Western Blot法检测基质金属蛋白酶-14(MMP-14)的表达。结果显示:miR-362-3p在前列腺癌组织中低表达。miR-362-3p在前列腺癌患者的脉管癌栓、Gleason分级、转移和不同TNM分期的表达存在显著差别。miR-362-3p与前列腺癌患者的生存时间显著相关,miR-362-3p分别与MMP-11、MMP-14具有显著负相关性,但与E-cadherin具有显著正相关性。结果表明,miR-362-3p可能通过调节细胞粘附和细胞外基质参与前列腺癌转移,可能作为患者的预后标志物。To explore the relationship between microRNA-362-3p(miR-362-3p)and metastasis in prostatic adenocarcinoma,prostatic adenocarcinoma were selected as observation group,and benign prostatic hyperplasia as control group(n=67).MiR-362-3p was detected by real-time fluorescence quantitative PCR(qPCR).Expressions of E-cadherin and MMP-11 were detected by immunohistochemistry method,MMP-14 was detected by Western Bloting.Result showed lower expression of miR-362-3p in the prostate adenocarcinoma,expression of miR-362-3p was significantly different in vascular tumor thrombus,gleason classification,metastasis and TNM stages.MiR-362-3p was correlated with survival time.Negative correlation was found between miR-362-3p and MMP-11 or MMP-14.Positive correlation was found between miR-362-3p and E-cadherin.The results suggested miR-362-3p may be involved in progression of prostate adenocarcinoma by regulating cell adhesion and extracellular matrix,and may be a prognostic marker.

关 键 词：前列腺癌 miR-362-3p 粘附素 细胞外基质 预后 

分 类 号：R73[医药卫生—肿瘤]