外周血单个核细胞Tim-3及其配体HMGB1在HBV相关肝癌诊断中的意义  

The significance of peripheral blood mononuclear cells Tim-3 and its ligand HMGB1 in the diagnosis of HBV-related hepatocellular carcinoma

作　　者：刘阔[1] 刘鹏 张晓宁[1] LIU Kuo;IIU Peng;ZHANG Xiao-ning(Department of Clinical Laboratory,The First Ailited Hospital of Shandong First Medical University(Shandong Provincial Qianfoshan Hospital),Shandong Jinan 250014)

机构地区：[1]山东第一医科大学第一附属医院(山东省千佛山医院)检验科,山东济南250014

出　　处：《医学检验与临床》2020年第3期1-4,共4页Medical Laboratory Science and Clinics

基　　金：国家自然科学基金青年项目81602087;山东省自然科学基金青年项目ZR2014HQ063;山东省医药卫生科技发展计划2016WS0476。

摘　　要：目的:检测Tim-3-HMGB1在HBV相关肝癌患者中的表达水平.方法:收集35例肝癌患者及40例健康对照外周血,采用Ficoll-Hypaque梯度离心法分离外周血单个核细胞(PBMC),分别提取血清病毒DNA及总RNA,普通聚合酶链反应(PCR)检测HBV-DNA、Tim3表达水平;实时荧光定量PCR检测HMGB1表达水平.结果:HBV相关肝癌患者外周血单个核细胞Tim-3(P<0.01)-HMGB1(P=0.019)表达水平均增高.结论:HBV相关肝癌患者外周血单个核细胞Tim-3-HMGB1表达水平增高,可作为辅助诊断指标应用.Objective:To detect the expression of tim-3-HMGB1 in patients with HBV related hepatocellular carcinoma(HCC).Methods:The peripheral blood of 35 patients with liver cancer and 40 healthy controls were collected.Peripheral blood mononuclear cells(PBMC)were isolated by Ficoll Hypaque gradient centrifugation,and virus DNA and total RNA were extracted respectively.HBV-DNA and tim3 expression levels were detected by polymerase chain reaction(PCR).HMGB1 expression was detected by real-time fluorescence quantitative PCR.Results:the mRNA expression level of Tim-3(P<0.01)-HMGB1(P=0.019)in PBMCs of patients with HBV related liver cancer were increased.Conclusion:The expression of tim-3-HMGB1 in PBMCs of patients with HBV related liver cancer could be used as an auxiliary diagnostic index.

关 键 词：肝细胞肝癌 乙型肝炎病毒 T淋巴细胞免疫球蛋白黏蛋白-3 高迁移率族蛋白B1 

分 类 号：R73[医药卫生—肿瘤]