分子印迹膜协同银增强电化学信号高灵敏特异性检测啶虫脒  

Molecular imprinting synergistic silver-enhanced electrochemical signal for highly sensitive and specific detection of acetamiprid

作　　者：严政 唐建设 YAN Zheng;TANG Jianshe(School of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601;Anhui Key Laboratory of Water Pollution Control and Wastewater Reuse,Anhui Jianzhu University,Hefei 230601)

机构地区：[1]安徽建筑大学环境与能源工程学院,合肥230601 [2]安徽建筑大学水污染控制与废水资源化安徽省重点实验室,合肥230601

出　　处：《分析试验室》2020年第2期161-165,共5页Chinese Journal of Analysis Laboratory

基　　金：浙江省科技厅分析测试项目(LGC19B050010);浙江树人大学实验室开放项目(2019JS1003)资助。

摘　　要：以啶虫脒为模板分子,壳聚糖为功能单体,戊二醛为交联剂,协同Ag颗粒增强导电性,在丝网印刷电极表面合成分子印迹膜,研制了测定啶虫脒的分子印迹传感器。通过循环伏安法(CV)对传感器的性能进行研究,在扫描速率100 mV/s的最优条件下,CV测试表明,啶虫脒浓度在0.01~1.0μmol/L的浓度范围内呈现良好的线性关系(线性相关系数R^2=0.9342)。分子印迹传感器具有良好的印迹效果,相较于其他农药如吡虫啉、水胺硫磷、乙草胺,对啶虫脒有较高的特异性识别能力。构建了啶虫脒分子印迹传感器的动力学吸附模型,测得传感器的印迹因子(β)为3.61,结合速率(k)为19.46 s。传感器用于水样中啶虫脒的测定,加标回收率在92.9%~100.4%。A molecularly imprinted membrane for the determination of acetamiprid was developed by synthesizing a molecularly imprinted membrane on the surface of a screen-printed electrode,using acetamiprid as a template molecule,chitosan as a functional monomer,glutaraldehyde as a cross-linking agent,and synergistic with Ag particles to enhance conductivity.The performance of the sensor was investigated by cyclic voltammetry(CV).Under the optimal conditions of scanning rate of 100 m V/s,the CV results showed that the concentration of acetamiprid had a good linear relationship in the concentration range of 0.01-1.0μmol/L(linear correlation coefficient R^2=0.9342).The molecularly imprinted sensor has good imprinting effect and higher specific recognition ability for acetamiprid than other pesticides such as imidacloprid,amphetamine,and acetochlor.The kinetic adsorption model of the molecularly imprinted sensor of acetamiprid was constructed.The imprinting factorβof 3.61 and the binding rate k of 19.46 s were measured.The sensor was used for the determination of acetamiprid in water samples with the recoveries of 92.9%-100.4%.

关 键 词：啶虫脒 丝网印刷电极 壳聚糖 Ag颗粒 分子印迹传感器 

分 类 号：X132[环境科学与工程—环境科学]