Nano-ZnO微叉指电极生物传感器检测大肠杆菌  

Nano-ZnO micro-interdigitied electrode biosensor for detecting E.coli O157:H7

作　　者：王春皓 崔传金 张学超[2] 谷学静 李静 WANG Chunhao;CUI Chuanjin;ZHANG Xuechao;GU Xuejing;LI Jing(School of Electrical Engineering,North China University of Science and Technology,Tangshan 063210;Tongren University,Tongren 554300)

机构地区：[1]华北理工大学电气工程学院,唐山063210 [2]贵州铜仁学院,铜仁554300

出　　处：《分析试验室》2020年第2期207-212,共6页Chinese Journal of Analysis Laboratory

基　　金：河北省教育厅项目(ZD2016070);河北省食品药品监督管理局项目(QN2015012);黔教合KY字[2018]342;黔科合LH字[2017]7328资助。

摘　　要：设计了一种基于纳米ZnO材料检测大肠杆菌(E.coli O157:H7)的微叉指阻抗生物传感器,利用电化学方法在氧化铟锡(ITO)叉指电极表面沉积上纳米ZnO,然后将链霉亲和素固定在纳米ZnO表面,利用生物素亲和素的高亲和性原理将大肠杆菌抗体绑定在传感器表面,完成传感器的构建。实验表明,传感器检测E.coli O157:H7线性范围为40~4×10^6cfu/mL,检出限为40 cfu/mL,传感器的特异性、重现性、实用性较好。A nano-ZnO-based micro-interdigited impedance biosensor was designed for detecting E.coli O157:H7.Nano-ZnO was deposited on the surface of ITO interdigited electrode by electrochemical method.Then streptavidin was immobilized on the surface of nano-ZnO,the biotinized antibody was fixed on the surface of the sensor based on the high affinity principle between biotin and avidin,and the sensor construction was completed.The results showed that the linear detection range was 40 cfu/m L to 4×10^6 cfu/m L,and the detection limit of the sensor was 40 cfu/m L.The sensor also showed good specificity,reproducibility and practicability.

关 键 词：纳米氧化锌 生物素与亲和素 生物传感器 大肠杆菌O157:H7 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]