慢性髓系白血病患者SHP-1基因、id4基因甲基化状态及其与疾病进展的关系  

Methylation of SHP-1 and ID4 genes and their relationship with disease progression in chronic myeloid leukemia

作　　者：郑伟 侯志新 刘承祥 张雷 韩松 Zheng Wei;Hou Zhixin;Liu Chengxiang;Zhang Lei;Han Song(Department of Pathology,The 964th Hospital of the PLA Joint Logistics Support Force, Changchun130062, China)

机构地区：[1]中国人民解放军联勤保障部队第九六四医院病理科,长春130062

出　　处：《疑难病杂志》2020年第5期496-499,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：吉林省卫生厅科技计划项目(2013ZC023)。

摘　　要：目的探究慢性髓系白血病(CML)患者含SH2结构域的磷酸酶1(SHP-1)基因、DNA结合抑制因子4(id4)基因甲基化状态及其与疾病进展的关系。方法选取2015年5月—2019年5月中国人民解放军联勤保障部队第九六四医院血液科收治CML患者78例为研究对象(病例组),选取同期于医院健康体检者42例作为健康对照组。使用甲基化特异性聚合酶链反应(MS-PCR)法对2组受试者骨髓中SHP-1基因、id4基因甲基化表达进行检测,分析CML患者慢性期、加速期及急变期SHP-1基因、id4基因甲基化率。结果SHP-1、id4基因mRNA表达水平比较,健康对照组>慢性期>加速期>急变期,差异均有统计学意义(F=83.813、424.699,P均=0.000)。健康对照组、CML慢性期、加速期及急变期的SHP-1基因甲基化阳性率分别为0%、25.00%、96.67%及100.00%,差异具有统计学意义(χ^2=98.127,P=0.000);而id4基因甲基化阳性率分别为0%、0%、66.67%及70.83%,差异亦具有统计学意义(χ^2=65.490,P=0.000)。结论SHP-1基因、id4基因在健康者中呈非甲基化,在CML慢性期甲基化率较低,在加速期或急变期则呈高甲基化状态,因而其甲基化可作为预测CML疾病进展的标志因子,对CML的诊断及治疗具有积极意义。Objective To explore the methylation status of phosphatase-1(SHP-1)gene and DNA binding inhibitor-4(ID4)gene with SH2 domain in patients with chronic myeloid leukemia(CML)and their relationship with disease progression.Methods From May 2015 to May 2019,78 CML patients(case group)were enrolled in the Hematology Department of the 964 Hospital of the Joint Service Support Force of the People's Liberation Army.42 healthy people in the same period were selected as the control group.The methylation expression of SHP-1 gene and ID4 gene in bone marrow of two groups of subjects was detected by methylation specific PCR.To analyze the methylation rate of SHP-1 gene and ID4 gene in CML patients in chronic,accelerated and acute phases.Results Compared with the expression level of SHP-1 and ID4 gene mRNA,the difference was statistically significant(F=83.813,424.699,P=0.000).The positive rates of SHP-1 gene methylation in healthy control group,CML in chronic phase,accelerated phase and acute phase were 0%,25.00%,96.67%and 100.00%,respectively,with statistical significance(χ^2=98.127,P=0.000);the positive rates of ID4 gene methylation were 0%,0%,66.67%and 70.83%,with statistical significance(χ^2=65.490,P=0.000).Conclusion SHP-1 gene and ID4 gene are unmethylated in healthy people,low methylation rate in chronic phase of CML,and high methylation rate in accelerated phase or acute phase of CML,so their methylation can be used as a marker to predict the progress of CML disease,which has positive significance for the diagnosis and treatment of CML.

关 键 词：慢性髓系白血病 含SH2结构域的磷酸酶1 DNA结合抑制因子4 甲基化状态 疾病进展 

分 类 号：R73[医药卫生—肿瘤]