TGF-βRⅡ、SCF、Cx-37基因和蛋白在卵巢早衰中的表达及意义  

Expression and significance of TGF-βRⅡ,SCF,Cx-37 genes and proteins in premature ovarian failure

作　　者：李振淼 章青 马妍 盛菲 李文 Li Zhenmiao;Zhang Qing;Ma Yan;Sheng Fei;Li Wen(Reproductive Medicine Center, Long March Hospital Affiliated to Naval Medical University of the people's Liberation Army of China, Shanghai 200000,China)

机构地区：[1]中国人民解放军海军军医大学附属长征医院生殖医学中心,上海200000

出　　处：《疑难病杂志》2020年第5期505-509,共5页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的探讨转化生长因子β受体Ⅱ(TGF-βRⅡ)、干细胞因子(SCF)、间隙连接蛋白37(Cx-37)在卵巢早衰(POF)中的表达及意义。方法2019年6月—9月在中国人民解放军海军军医大学附属长征医院动物实验室进行实验,将雌性大鼠40只按随机数字表法分为实验组和对照组,各20只。实验组大鼠予雷公藤多苷片悬浮液50 mg·kg^-1·d^-1,1次/d,共2周,制作POF模型;对照组大鼠给予上述相同剂量的生理盐水灌胃,1次/d,共2周。之后抽取血液并处死,同时留存大鼠卵巢。卵巢切片后HE染色,PCR法测定TGF-βRⅡ、SCF、Cx-37 mRNA表达,Western-Blot法测定TGF-βRⅡ、SCF、Cx-37蛋白表达。结果实验组大鼠成熟卵泡、次级卵泡及初级卵泡明显少于对照组,闭锁卵泡明显多于对照组,差异均有统计学意义(t/P=19.899/0.000,10.407/0.000,8.364/0.000,18.491/0.000);血清E2水平低于对照组,血清FSH水平高于对照组(t/P=16.067/0.000,37.947/0.000);TGF-βRⅡ、Cx-37 mRNA和蛋白表达水平低于对照组,SCF mRNA和蛋白表达水平高于对照组,差异均有统计学意义(t/P=12.642/0.000,12.081/0.000,69.397/0.000,50.447/0.000,33.001/0.000,48.152/0.000)。结论卵巢早衰大鼠的SCF蛋白呈现高水平表达,TGF-βRⅡ、Cx-37蛋白呈现低水平表达,在卵巢早衰疾病的发生发展过程中起着重要的作用,可为减缓卵巢早衰提供新的临床指标和靶向治疗思路。Objective To investigate the expression and significance of transforming growth factorβreceptorⅡ(TGF-βRⅡ),stem cell factor(SCF)and gap junction protein 37(Cx-37)in premature ovarian failure(POF).Methods From June to September 2019,the experiment was conducted in the Animal Laboratory of Changzheng Hospital Affiliated to the Naval Medical University of the People's Liberation Army.40 female rats were divided into two groups according to the random number table:the experimental group and the control group.The rats in the experimental group were given 50 mg·kg^-1·d^-1 of Tripterygium glycoside suspension once a day for 2 weeks to make POF model.Rats in the control group were given the same dose of saline once a day for 2 weeks.After that,blood was drawn and killed,and the ovaries of rats were preserved.The expression of TGF-βRⅡ,SCF and Cx-37 mRNA was detected by PCR and Western blot respectively.Results The number of mature follicles,secondary follicles and primary follicles in the experimental group was significantly lower than that in the control group,and the number of atretic follicles was significantly higher than that in the control group(t/P=19.899/0.000,t/P=10.407/0.000,t/P=8.364/0.000,t/P=18.491/0.000).The level of serum E2 was lower than the control group,the level of serum FSH was higher than the control group(t/P=16.067/0.000,t/P=37.947/0.000),the level of TGF-βRⅡ,Cx-37 mRNA and protein expression was lower than the control group,the level of SCF mRNA and protein expression was higher than the control group,the difference was statistically significant(t/P=12.642/0.000,t/P=12.081/0.000,t/P=69.397/0.000,t/P=50.447/0.000,t/P=33.001/0.000,t/P=48.152/0.000).Conclusion The expression of SCF protein and TGF-βRⅡand Cx-37 protein in rats with premature ovarian failure is high level,which play an important role in the development of premature ovarian failure.It can provide new clinical indicators and targeted treatment ideas for the alleviation of premature ovarian failure.

关 键 词：卵巢早衰 转化生长因子β受体Ⅱ 干细胞因子 间隙连接蛋白37 大鼠 

分 类 号：R73[医药卫生—肿瘤]