ILK、β-catenin和VEGF在非小细胞肺癌中的表达及意义  

Expression and significance of ILK,β-catenin and VEGF in non-small cell lung cancer

作　　者：王林辉[1] 陈慧勇[2] 杜日昌[1] 李静[1] 肖高芳[1] 高双全[1] 张莹 WANG Linhui;CHEN Huiyong;DU Richang;LI Jing;XIAO Gaofang;GAO Shuangquan;ZHANG Ying(Department of Pathology,Yuebei People′s Hospital,Shaoguan,Guangdong 512026,China;Department of Thoracic Surgery,Yuebei People′s Hospital,Shaoguan,Guangdong 512026,China)

机构地区：[1]广东省韶关市粤北人民医院病理科,512026 [2]广东省韶关市粤北人民医院胸外科,512026

出　　处：《重庆医学》2020年第9期1433-1437,共5页Chongqing medicine

基　　金：广东省韶关市卫生和计划生育局科研课题(Y19037)。

摘　　要：目的探讨整合素连接激酶(ILK)、β-连环蛋白(β-catenin)和血管内皮生长因子(VEGF)在非小细胞肺癌(NSCLC)组织中的表达水平及其与临床病理特征间的关系。方法应用免疫组织化学SP法测定108例NSCLC患者癌组织(NSCLC组)、癌旁组织(距离肿瘤2 cm,癌旁组)和正常肺组织(距离肿瘤5 cm,对照组)中ILK、β-catenin及VEGF的表达水平,并对其表达水平与患者的性别、年龄、肿瘤体积大小、病理类型、分化程度、淋巴结转移、远处器官转移和临床TNM分期等情况进行分析,同时对β-catenin、ILK和VEGF之间进行相关性分析。结果免疫组化结果显示β-catenin阳性表达主要位于细胞核,而ILK和VEGF的阳性表达主要位于细胞质。NSCLC组中β-catenin、ILK和VEGF的阳性表达率分别为68.52%(74/108)、75.93%(82/108)和85.19%(92/108),明显高于癌旁组和对照组(P<0.05)。ILK、β-catenin和VEGF的阳性表达与NSCLC的分化程度、远处器官转移、淋巴结转移和TNM分期明显相关(P<0.05);与患者年龄、性别、瘤体大小、病理类型无关(P>0.05)。在NSCLC组中,β-catenin的阳性表达和ILK的阳性表达呈明显正相关(P<0.05),ILK的过度表达和VEGF的阳性表达也呈明显正相关(P<0.05)。结论ILK、β-catenin和VEGF在NSCLC中高表达且相互影响,对其浸润深度、淋巴结转移和远处器官转移等都有着重要作用。Objective To investigate the expression levels of intergrin-linked kinase(ILK),β-catenin and vascular endothelial growth factor(VEGF)in the tissue of non-small cell lung cancer(NSCLC)and their relation with clinicopathological features.Methods The immunohistochemical(IHC)SP method was applied to detect the expression levels of ILK,β-catenin and VEGF in cancer tissues(NSLC group),paracancerous tissues(2 cm from tumor,paracancerous group)and normal pulmonary tissue(5 cm from tumor,control group)of 108 cases of NSCLC.The relationships between the expression levels of ILK,β-catenin and VEGF with the sex,age,tumor size,pathological type,differentiation degree,lymph node metastasis,distant organs metastasis and clinical TNM stage were analyzed.Meanwhile the correlation of ILK,β-catenin and VEGF was also analyzed.Results The IHC results showed that the positive expression ofβ-catenin was mainly in the nucleus,while the positive expression of ILK and VEGF was mainly located in the cytoplasm.The positive expression rates ofβ-catenin,ILK and VEGF in the NSCLC group were 68.52%(74/108),75.93%(82/108)and 85.19%(82/108)respectively,which were significantly higher than those in the paracancerous group and control group(P<0.05).The positive expressions ofβ-catenin,ILK and VEGF had significant correlation with the differentiation degree,distant organ metastasis,lymph node metastasis and TNM stage of NSCLC(P<0.05),but had no correlation with the age,sex,tumor size and pathological type(P>0.05).In the NSCLC group,the positive expression ofβ-catenin had significantly positive correlation with the ILK positive expres-sion(P>0.05),and the overexpression of ILK also had significantly positive correlation with the VEGF positive expression(P>0.05).Conclusionβ-catenin,ILK and VEGF in NSCLC are highly expressed,interacted with each other and play the important role in the invasion depth,lymph node metastasis and distant organ metastasis of NSCLC.

关 键 词：癌 非小细胞肺 Β-连环蛋白 整合素连接激酶 血管内皮生长因子 

分 类 号：R734.2[医药卫生—肿瘤]