Feeding effects of dsNPF interference in Ostrinia furnacalis  

作　　者：CUI Hong-ying WANG Yuan PENG Xin WANG Yi-tong ZHAO Zhang-wu 

机构地区：[1]Department of Entomology/MOA Key Lab of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第6期1475-1481,共7页农业科学学报（英文版）

基　　金：This work is supported by the National Natural Science Foundation of China(31730076).

摘　　要：The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrinia furnacalis.Results showed that the expression peaks of npfmRNA in both midgut and fat body are at 28 h of early 5th instar larvae.When NPF was kno eked dow n either by feedi ng dsNPF-c ontaining artificial diet or direct dsNPF in jection into the 5th in star larvae,npf expression was effectively inhibited in the midgut.Larval feeding,body weight and development time were significantly impacted.In con trast,the expressi on of the npf receptors npfrl and npfr2 in fat body of 5th in star larvae was sign ifica ntly increased by dsNPF injection.These results indicate that NPF can be effectively knoeked down in 0.furnacalis,and dsNPF by injection is an effective and fast way to silence npf expression.This study provides a critical basis for further exploration in mechanism of feeding regulation in 0.furnacalis.

关 键 词：NEUROPEPTIDE NPF NPFR FEEDING regulation ASIAN CORN BORER 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]