3种培养基运用于饮料无菌冷灌装生产线无菌验证效果的评价  

Evaluation on aseptic verification effect of three media used in beverage aseptic cold filling production line

作　　者：杨宁[1] 卢勉飞[1] 蔡芷荷[1] 邱国建[1] 容艳芬[1] 吴清平[2] YANG Ning;LU Mian-fei;CAI Zhi-he;QIU Guo-jian;RONG Yan-fen;WU Qing-ping(Guangdong Huankai Microbial Sci.&Tech.Co.Ltd.,Guangzhou,Guangdong 510663,China)

机构地区：[1]广东环凯微生物科技有限公司,广东广州510663 [2]广东省微生物研究所,广东广州510070

出　　处：《中国卫生检验杂志》2020年第8期906-908,915,共4页Chinese Journal of Health Laboratory Technology

基　　金：广东省重大科技专项(2015B020230006);广州市产学研协同创新重大专项(201604016068)。

摘　　要：目的通过比较LG培养基(LG)、胰酪胨大豆肉汤(TSB)和改良马丁培养基(MMB)对细菌和真菌的检测灵敏度和促生长能力,评价LG培养基是否适用于PET瓶无菌冷灌装生产线的无菌验证。方法选用4株细菌和2株真菌分别对LG、TSB和MMB培养基的检测灵敏度和促生长能力进行评估。结果4种细菌在LG与TSB中的检测灵敏度相当,两者略优于MMB;2种真菌在LG与MMB中的检测灵敏度与促生长能力相当,两者略优于TSB。结论LG培养基无论对细菌还是真菌,均具有较高的检测灵敏度及较优的促生长能力,而且该培养基成本比较低,非常适用于需要使用大量培养基的无菌冷灌装生产线的无菌验证。Objective By comparing the sensitivity and the capacity of promoting the growth of bacteria and fungi among Linden Grain(LG)medium,Tryptic-soy-broth(TSB)medium and modified martin(MMB)medium,to evaluate whether the LG medium is suitable for sterility test of aseptic PET bottle cold filing line.Methods Four strains of bacteria and two strains of fungi were selected to evaluate the sensitivity and growth-promoting ability of LG,TSB and MMB culture media.Results The sensitivity of LG medium were same as that of TSB medium for four kinds of bacteria,two of them are slightly better than MMB.The sensitivity and the capacity of promoting the growth of two kinds of fungi of LG medium were same as that of MMB medium,two of them are slightly better than TSB.Conclusion LG medium is very suitable for the sterility test of aseptic cold filling production line which needs a large number of medium,for it has higher detection sensitivity and better growth promoting ability for bacteria and fungi,and relatively low cost.

关 键 词：LG培养基 胰酪胨大豆肉汤 无菌冷灌装生产线 无菌验证 对比检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]