CD39在头颈部鳞状细胞癌组织中的表达及其预后价值  

Expression of CD39 in head and neck squamous cell carcinoma tissues and its prognostic value

作　　者：王学周 周莉 李百会 胡雪 董瑞峰 张新伟 WANG Xuezhou;ZHOU Li;LI Baihui;HU Xue;DONG Ruifeng;ZHANG Xinwei(Biotechnology Lab,National Clinical Research Center for Cancer,Key Laboratory of Cancer Immunology and Biotherapy of Tianjin City,Key Laboratory of Cancer Prevention and Therapy of Tianjin City,Tianjin Clinical Research Center for Malignant Cancer,Cancer Hospital of Tianjin Medical University,Tianjin 300060,China)

机构地区：[1]天津医科大学肿瘤医院/生物技术研究室/国家肿瘤临床医学研究中心/天津市肿瘤免疫与生物治疗重点实验室/天津市“肿瘤防治”重点实验室/天津市恶性肿瘤临床医学研究中心,天津300060

出　　处：《中国肿瘤生物治疗杂志》2020年第4期396-402,共7页Chinese Journal of Cancer Biotherapy

基　　金：国家科技支撑计划资助项目(No.2015BAI12B00);天津市卫生行业重点攻关项目(No.15KG145)。

摘　　要：目的:检测CD39在头颈鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织中的表达,分析其表达与患者临床病理特征的关系及其预后意义。方法:选用2012年5月至2013年12月在天津市肿瘤医院接受外科手术的85例HNSCC患者的组织标本及病例资料,Oncomine数据库获取的基因芯片,以及HNSCC细胞系SCC15、UM1和Cal25。在线分析CD39在HNSCC组织与正常颊黏膜组织转录水平的差异性,用Western blotting和免疫组化法检测HNSCC组织中CD39蛋白的表达。采用Spearman’s检验分析HNSCC组织中CD39的表达与患者临床病理特征的相关性,Kaplan-Meier曲线法和Log rank检验分析HNSCC组织CD39表达与生存的关系,Cox风险比例回归模型评价CD39表达与复发风险的关系。结果:CD39在HNSCC组织的转录水平显著高于正常颊黏膜组织(P<0.01),其在HNSCC细胞Cal25、SCC-15和UM1中均有表达,UM1细胞中CD39的表达呈地塞米松(dexamethasone,DXM)剂量依赖性。CD39高表达患者53(62.4%)例,其高表达与术前化疗正相关(r=0.234,P<0.05),CD39高表达患者的无复发生存期较低表达组显著缩短(P<0.05),CD39高表达是HNSCC复发的独立风险因素(HR=2.328,95%CI=1.091~4.967;P<0.05)。结论:CD39在HNSCC中呈DXM诱导性表达和组成性表达,其在癌组织中过表达是HNSCC患者不良预后的独立预测因子,提示其在HNSCC进展过程中可能发挥重要作用。Objective: To detect the expression of CD39 in head and neck squamous cell carcinoma(HNSCC) tisseus, and to analyze its correlation with patients’ clinicopathological features and its prognostic significance. Methods: Tissue specimens and case data of85 patients with HNSCC underwent surgery at Cancer Hospital of Tianjin from May 2012 to December 2013 were collected for this study. Gene chips were obtained from Oncomine database, and HNSCC cell lines SCC15, UM1, and Cal25 were selected for this study.Online analysis was performed to compare the differential expression of CD39 in buccal mucosa(BM) tissues and HNSCC tissues,Western blotting and Immunohistochemistry(IHC) were used to detect the protein expression of CD39 in HNSCC tissues. Spearman’s correlation analysis was used to study the correlation between the expressions of CD39 and clinicopathological features of HNSCC patients. Both Kaplan-Meier curve analysis and Log rank test were used to analyze the association between the expression of CD39 in HNSCC tissues and the survival of patients, and Cox risk proportional regression model was used to evaluate the relationship between CD39 expression and the risk of relapse. Results: The transcription level of CD39 was obviously up-regulated in HNSCC tissues than in BM tissues(P<0.01), and CD39 expression was detected in HNSCC cell lines SCC15, UM1 and Cal25. Dexamethasone(DXM)could enhance the expression of CD39 in UM1 cells in dose-dependent manner. CD39 was highly expressed in 53(62.4%) HNSCC patients, which was positively correlated with preoperative chemotherapy(r=0.234, P<0.05). The recurrence-free survival(RFS) of patients with high CD39 expression was significantly shortened(P<0.05), and high CD39 expression was an independent relapse risk factor(HR=2.328, 95%CI=1.091-4.967;P<0.05) for patients with HNSCC. Conclusion: CD39 is DXM-inducively and constitutively expressed in HNSCC. And over-expression of CD39 is an independent predictor of poor prognosis in HNSCC patients, indicating its important role in t

关 键 词：头颈鳞状细胞癌 UM1细胞 CD39 术前化疗 地塞米松 预后 

分 类 号：R739.5[医药卫生—肿瘤] R730.7[医药卫生—临床医学]