lncRNA DGCR5在食管鳞状细胞癌组织中的表达及其临床意义  

Expression of lncRNA DGCR5 in esophageal squamous cell carcinoma tissues and its clinical significance

作　　者：段玉青[1] 王梦杰 王洪琰[2] 王郁[1] 桑梅香[3] 刘丽华[1] DUAN Yuqing;WANG Mengjie;WANG Hongyan;WANG Yu;SANG Meixiang;LIU Lihua(Department of Tumor Immunotherapy,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050035,Hebei,China;Department of Chest Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050035,Hebei,China;Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050035,Hebei,China)

机构地区：[1]河北医科大学第四医院肿瘤免疫科,河北石家庄050035 [2]河北医科大学第四医院胸外科,河北石家庄050035 [3]河北医科大学第四医院科研中心,河北石家庄050035

出　　处：《中国肿瘤生物治疗杂志》2020年第4期416-419,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.81871894)。

摘　　要：目的:探讨长链非编码RNA DiGeorge综合征临界区基因5(digeorge syndrome critical region gene 5,DGCR5)在食管鳞状细胞癌(esophageal squamous cell cancinoma,ESCC)组织中的表达及其与患者临床病理特征和预后的相关性。方法:利用生物信息学方法对TCGA数据库中ESCC数据集的DGCR5表达进行分析。收集2016年8月至2017年3月河北医科大学第四医院手术切除60例ESCC患者的癌及癌旁组织标本,用qPCR检测ESCC组织中DGCR5的表达水平,分析DGCR5表达与ESCC患者临床病理特征和预后的相关性。结果:TCGA数据库分析结果显示,DGCR5在ESCC组织中的表达水平显著高于正常食管组织(P<0.01)。ESCC组织中DGCR5表达水平显著高于癌旁组织(P<0.01)。DGCR5表达水平与ESCC患者TNM分期和淋巴结转移呈显著性相关(均P<0.05)。Kaplan-Meier单因素分析显示,高表达DGCR5的ESCC患者2年生存率显著低于低表达DGCR5患者(P<0.05)。结论:DGCR5在ESCC组织中呈高表达状态,共表达与TNM分期、淋巴结转移及预后不良密切相关,可能成为ESCC早期诊断及预后判断的分子标志物。Objective: To investigate the expression of long non-coding RNA(lncRNA) DiGeorge syndrome critical region gene 5(DGCR5) in esophageal squamous cell carcinoma(ESCC) tissues, and to analyze its relationship with clinicopathological features and prognosis of ESCC patients. Methods: The expression of DGCR5 in ESCC data set from TCGA database was analyzed by bioinformatics method. Sixty pairs of ESCC tissues and para-cancerous tissues resected at the Fourth Hospital of Hebei Medical University from August 2016 to March 2017 were collected for this study. The expression of DGCR5 in ESCC tissues was detected by qPCR. The correlation between the expression of DGCR5 and the clinicopathological features and prognosis of ESCC patients was analyzed. Results: TCGA database analysis showed that the expression of DGCR5 in ESCC tissues was significantly higher than that in normal esophageal tissues(P<0.01). The expression of DGCR5 in ESCC tissues was significantly higher than that in para-cancerous tissues(P<0.01). The expression level of DGCR5 was significantly correlated with TNM staging and lymph node metastasis in ESCC patients(all P<0.05). Kaplan-Meier univariate analysis showed that the 2-year survival rate of ESCC patients with high DGCR5 expression was significantly lower than that of patients with low expression(P<0.05). Conclusion: DGCR5 is highly expressed in ESCC tissues and is closely related to TNM staging, lymph node metastasis and poor prognosis, which may serve as a molecular marker for early diagnosis and prognosis prediction of ESCC.

关 键 词：食管鳞状细胞癌 长链非编码RNA DiGeorge综合征临界区基因5 TCGA数据库 生存分析 

分 类 号：R735.1[医药卫生—肿瘤] R730.7[医药卫生—临床医学]