microRNA-182-5p靶向BNIP3抑制卵巢癌细胞增殖和迁移的研究  

Inhibition of proliferation and migration of ovarian cancer cells by microRNA-182-5p targeting BNIP3

作　　者：李芳芳[1] 陈莉[1] 张先娟[1] 张传厚[1] 顾振鹏[1] LI Fang-fang;CHEN Li;ZHANG Xian-juan;ZHANG Chuan-hou;GU Zhen-peng(Department of Gynecology,Binzhou Medical University Hospital,Binzhou,Shandong Province,256603,China)

机构地区：[1]滨州医学院附属医院妇科,山东滨州256603

出　　处：《中国临床药理学杂志》2020年第7期792-795,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨microRNA-182-5p(miR-182-5p)对卵巢癌SKOV3细胞增殖和迁移的抑制作用及其作用机制。方法将对数生长期SKOV3细胞随机分为空白组、对照组和实验组,并分别转染空白试剂、miR-182-5p拟似物、miR-182-5p+LV-BNIP3。转染后24 h,用噻唑蓝法检测细胞的增殖活性,用逆转录聚合酶链反应法检测miR-182-5p的表达水平,用Western Blotting法检测BNIP3蛋白的表达水平,用Transwell小室法检测细胞的迁移能力。结果转染后24 h,实验组、对照组和空白组的增殖活性(OD值)分别为(0.38±0.04),(0.17±0.03)和(0.39±0.02),miR-182-5p表达水平分别为(2.55±0.11),(2.52±0.13)和(0.98±0.11),BNIP3蛋白表达水平分别为(0.99±0.12),(0.42±0.11)和(0.96±0.09),穿膜细胞数分别为(138±29),(64±18)和(132±23)个,对照组的上述指标与实验组和空白组比较,差异均有统计学意义(均P<0.05)。结论过表达miR-182-5p对卵巢癌SKOV3细胞具有抑制作用,BNIP3可能是miR-182-5p调节卵巢癌SKOV3细胞增殖、迁移的作用靶点。Objective To investigate the inhibitory effect of microRNA-182-5 p(miR-182-5 p) on the proliferation and migration of SKOV3 cells and its mechanism.Methods The SKOV3 cells were randomly divided into blank group,control group and experimental group, and the blank reagent, miR-182-5 p mimics, miR-182-5 p mimics+LV-BNIP3 were transfected according to the instructions of Lipofectamine 2000, respectively.At 24 hours after transfection, the proliferation activity of the cells was detected by thiazole blue method, expression level of miR-182-5p was detected by reverse transcription polymerase chain reaction, the expression level of BNIP3 was detected by Western Blotting method, and the migration ability of the cells was detected by Transwell chamber method.Results At 24 hours after transfection, the proliferative activities(OD value) in experimental, control and blank groups were(0.38±0.04),(0.17±0.03) and(0.39±0.02), the expression levels of miR-182-5p were(2.55±0.11),(2.52±0.13) and(0.98±0.11), the expression levels of BNIP3 protein were(0.99±0.12),(0.42±0.11) and(0.96±0.09),the number of membrane penetrating cells were(138±29),(64±18) and(132±23).The above-mentioned indexes in the control group were significantly different from those in the experimental group and the blank group(all P < 0.05).Conclusion Over expression of miR-182-5 p can inhibit the proliferation and migration of ovarian cancer SKOV3 cells and BNIP3 may be the target of miR-182-5 p in regulating the proliferation and migration of ovarian cancer SKOV3 cells.

关 键 词：卵巢癌 microRNA-182-5p Bcl2和腺病毒E1B 19 kDa相互作用蛋白3 

分 类 号：R97[医药卫生—药品]