远隔缺血处理对大鼠脑组织TFEB-溶酶体功能影响的研究  

Effects of remote ischemic preconditioning on TFEB-lysosoms function in rat brain

作　　者：李志伟[1] 崔小丽[1] 刘杨[2] 刘小玲[1] 吕桦[1] 刘军[1] Li Zhiwei;Cui Xiaoli;Liu Yang;Liu Xiaoling;Lv Hua;Liu Jun(Department of Neurology,the Shanxi Provincial People’s Hospital,Xian 710068,Chian)

机构地区：[1]陕西省人民医院神经内一科,西安710068 [2]陕西省人民医院中心实验室,西安710068

出　　处：《脑与神经疾病杂志》2020年第5期305-309,共5页Journal of Brain and Nervous Diseases

基　　金：陕西省科学技术研究项目(2014K11-03-02-02)。

摘　　要：目的观察远隔缺血处理(RIPC)对大鼠脑组织转录因子EB(TFEB)-溶酶体功能的影响。方法SD大鼠随机分为3组:对照组、RIPC 24h组和RIPC 10d组。1次RIPC为3个循环的大鼠双后肢缺血-再灌注处理,每个循环缺血10 min,再灌注10 min。RIPC 24h组给予RIPC 1次,RIPC 10d组给予RIPC1次.d^-1,共10d。RIPC处理结束后留取大鼠血清,ELISA进行组织蛋白酶B(CTSB)测定,取出大鼠脑组织,ELISA进行CTSB测定,Western-blot进行溶酶体相关膜蛋白-1(LAMP-1)、TFEB测定。结果三组大鼠血清CTSB无差异,与对照组比较,RIPC 24h组大鼠脑组织CTSB以及细胞核内TFEB的含量明显增加,差异具有统计学意义(P<0.05)。RIPC 10d组大鼠进一步增加。与对照组相比,RIPC 24h组大鼠脑组织LAMP-1的含量有增加趋势,但差异无统计学意义;RIPC 10d组大鼠脑组织LAMP-1的含量明显增加,差异具有统计学意义(P<0.05)。结论RIPC可能通过激活脑组织TFEB,促进大鼠脑组织溶酶体相关蛋白的表达,增强溶酶体功能。Objective To investigate the effects of remote ischemic preconditioning(RIPC)on TFEB-lysosoms function in rat brain.Methods SD rats were randomly divided into 3 groups:Control group,RIPC 24 h group and RIPC 10 d group.One RIPC consist of 3 ischemia-reperfusion cycles of rat bilateral limbs,Ischemia for 10 minutes then reperfusion for 10 minutes.Rat in RIPC 24 h group were treated with one RIPC,and rat in RIPC 10 d group were treated with one RIPC/d for 10 days.CTSB in serums and CTSB,LAMP-1,TFEB in brain were measured by ELISA or Western-blot respectively.Results There was no significant difference in serums CTSB among 3 group.Compared to control group,Brain CTSB and nucleus TFEB in RIPC 24 h group increased significantly(P<0.05),and that of RIPC 10 d group further increased.Brain LAMP-1 in RIPC 24 h group has an increasing trend,but without statistical significance;Brain LAMP-1 in RIPC 10 d group increased significantly(P<0.05).Conclusion RIPC probably activate TFEB and promote expression of lysosomerelated proteins,then enhance lysosome function in rat brain.

关 键 词：远隔缺血处理 大鼠 组织蛋白酶B 溶酶体相关膜蛋白-1 转录因子EB 

分 类 号：R743.32[医药卫生—神经病学与精神病学]