卵巢肿瘤组织中CXCL9 mRNA表达与患者的预后、免疫微环境特征的相关性研究  

CXCL9 mRNA expression in ovarian tumor tissue and its relations with prognosis and characteristics of immune microenvironment

作　　者：高境泽 吴霞[1] GAO Jing-ze;WU Xia(Shanghai Key Laboratory of Gynecologic Oncology,Department of Obstetrics&Gynaecology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]上海交通大学医学院附属仁济医院妇产科,上海市妇科肿瘤重点实验室,上海200127

出　　处：《上海交通大学学报（医学版）》2020年第4期457-463,共7页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81472843)。

摘　　要：目的·探讨趋化因子CXCL9(C-X-C motif chemokine ligand 9)mRNA表达水平对卵巢癌患者总体生存期的影响,挖掘其与肿瘤微环境中关键免疫相关通路及基因表达的关联性,以揭示其在卵巢癌预后的重要意义。方法·采用Kaplan-Meier法分析CXCL9 mRNA表达与癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中卵巢癌患者总体生存期之间的关系。使用基因集富集分析(Gene Set Enrichment Analysis,GSEA)研究CXCL9 mRNA在卵巢癌中的生物学功能,并分析其与细胞分化抗原8A(cluster of differentiation 8A,CD8A)及免疫检查点的mRNA表达的相关性。结果·CXCL9 mRNA高表达与卵巢癌患者较好的预后显著相关。GSEA结果提示,CXCL9 mRNA富集于免疫反应相关通路。此外,Pearson相关分析显示CXCL9 mRNA表达与CD8A及免疫检查点mRNA表达呈正相关。结论·卵巢肿瘤组织中CXCL9 mRNA高表达是患者预后良好的预测指标,且CXCL9 mRNA表达可能与肿瘤组织中募集淋巴细胞至肿瘤微环境发挥抗肿瘤免疫应答功能密切相关。Objective·To investigate the effects of CXCL9(C-X-C motif chemokine ligand 9)mRNA expression on the overall survival of ovarian cancer patients,and to explore its relations with immune-related pathways and gene expression in tumor microenvironment,so as to reveal the significance of CXCL9 in the prognosis of ovarian cancer.Methods·Kaplan-Meier method was used to analyze the relationship between CXCL9 mRNA expression and the survival of ovarian cancer patients in The Cancer Genome Atlas(TCGA)database.Gene Set Enrichment Analysis(GSEA)was used to assess the biological function of CXCL9 mRNA in ovarian cancer.The correlation of CXCL9 mRNA with cluster of differentiation 8A(CD8A)and immune checkpoint mRNA expression was analyzed.Results·The high expression of CXCL9 mRNA was significantly associated with better prognosis in patients with ovarian cancer.GSEA showed that CXCL9 mRNA was enriched in the immune response-related pathway.In addition,Pearson correlation analysis showed that CXCL9 mRNA expression was positively correlated with the mRNA expression of CD8A and immune checkpoint.Conclusion·The high expression of CXCL9 mRNA in ovarian tumor tissue is a good predictor of prognosis,and the mRNA expression of CXCL9 may be closely related to the recruitment of lymphocytes from tumor margin to the tumor microenvironment to exert the function of anti-tumor immune response.

关 键 词：免疫微环境 CXCL9 卵巢癌 生物信息学分析 癌症基因组图谱 

分 类 号：R737.31[医药卫生—肿瘤]