P62基因对恶性黑色素瘤细胞侵袭的影响及机制研究  

Effect and mechanism study of P62 in malignant melanoma A375 cells invasion

作　　者：易娟娟[1] 招玉玲[1] 谢蒲辉[1] 唐妍[1] 黄惠珍[1] 郑丽 蔡艳桃[1] 杨竞 张晶 毛越苹[2] Juan-juan Yi;Yu-ling Zhao;Pu-hui Xie;Yan Tang;Hui-zhen Huang;Li Zheng;Yan-tao Cai;Jing Yang;Jing Zhang;Yue-ping Mao(Department of Dermatology,Foshan Women and Children Hospital,Foshan,Guangdong 528000,China;Department of Dermatology,the Memorial Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510120,China)

机构地区：[1]佛山市妇幼保健院皮肤科,广东佛山528000 [2]中山大学孙逸仙纪念医院皮肤科,广东广州510120

出　　处：《中国现代医学杂志》2020年第10期14-17,共4页China Journal of Modern Medicine

摘　　要：目的探讨P62基因在恶性黑色素瘤A375细胞迁移、侵袭中的作用及其机制。方法取对数生长期的恶性黑色素瘤A375细胞,采用干扰P62基因表达的siRNA及阴性对照siRNA分别转染A375细胞。采用Transwell法检测干扰组及对照组的细胞迁移、侵袭能力,Western blotting检测干扰组及对照组Wnt/β-catenin信号通路的表达。结果干扰组细胞Transwell迁移、侵袭能力较对照组下降(P<0.05);且干扰组Wnt/β-catenin及下游通路因子(P62、ZEB1、β-catenin、TCF4、c-Myc、c-Jun及CCND1)均受抑制(P<0.05)。结论P62可能通过调控Wnt/β-catenin及下游通路促进黑色素瘤细胞的迁移、侵袭。Objectives To explore the role and mechanism of P62 in malignant melanoma A375 cells invasion.Methods Malignant melanoma A375 cells were cultured at logarithmic growth phase,then they were transfected with siRNA-P62 and siNC,respectively.Transwell assays were both performed to detect the cell migration and invasion in interfering group and negative control group,respectively;Western Blot were both used to detect the expression of Wnt/β-catenin signaling pathways in two groups,respectively.Results The ability of cell migration and invasion decreased in interfering group compared with that in negative control group using Transwell(P<0.05),meanwhile,Wnt/β-catenin pathway and downstream factors(includingβ-catenin,TCF4,ZEB1,c-Myc,c-Jun,CCND1,and so on)were all inhibited in interfering gourp compared with that in negative control group(all P<0.05).Conclusions P62 may promote A375 cell migration and invasion via Wnt/β-catenin signaling pathways.

关 键 词：黑色素瘤 实验性 癌基因 肿瘤浸润 

分 类 号：R739.5[医药卫生—肿瘤]