八探针荧光原位杂交技术联合G显带技术在儿童急性淋巴细胞白血病中的应用  

Eight-probe fluorescence in situ hybridization combined with G-banding technique in children with acute lymphoblastic leukemia

作　　者：黄燕[1] 刘勇[1] 沈妙娜 陈海雷 黄倩雯 阮嘉钰 林晓英 Huang Yan;Liu Yong;Shen Miaona;Chen Hailei;Huang Qianwen;Ruan Jiayu;Lin Xiaoying(Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China)

机构地区：[1]中山大学孙逸仙纪念医院,广州510120

出　　处：《国际医药卫生导报》2020年第9期1224-1228,共5页International Medicine and Health Guidance News

摘　　要：目的探讨八探针荧光原位杂交(fluorescence in situ hybridization,FISH)联合G显带染色体核型分析在儿童急性淋巴细胞白血病(acute lymphoblastic leukemia)中的应用价值。方法应用八探针荧光原位杂交技术(CMYC、P16、E2A、CHIC2/D10Z1/D17Z1、TEL/AML1、MLL、BCR/ABL与IGH的DNA探针)和G显带染色体核型分析技术,对116例ALL患儿进行了联合检测。结果八探针FISH技术共检出75例患儿细胞遗传学改变,阳性率为64.65%,包括P16、E2A、CHIC2/D10Z1/D17Z1、TEL/AML1、MLL、BCR/ABL与IGH 7种细胞遗传学异常,但CMYC未检出。而G显带核型分析仅检出31例细胞遗传学异常,阳性率为26.72%。两种方法检出阳性率的差异有统计学意义(P<0.05)。结论八探针FISH技术较G显带染色体核型分析具有高效、省时、灵敏等优点。但G显带染色体核型分析检出面更为广泛,两者侧重面不同,与荧光原位杂交分析有效互补,两种技术相互补充可以提高检测效率,为临床个体化治疗提供更多的依据。Objective To investigate eight-probe fluorescence in situ hybridization(FISH)and G-banding karyotype analysis in the diagnosis of children with acute lymphoblastic leukemia(ALL).Method 116 children with ALL were tested by the eight-probe FISH(using probes for CMYC,P16,E2A,CHIC2/D10Z1/D17Z1,TEL/AML1,MLL,BCR/ABL,and IGH)and G-banding karyotype analysis.Results Cytogenetic changes were detected in 75(64.65%)children of all cases by the eight-probe FISH,involving P16,E2A,CHIC2/D10Z1/D17Z1,TEL/AML1,MLL,BCR/ABL,and IGH,but CMYC not detected.Cytogenetic changes were detected in 31(26.72%)children of all cases by G-banding karyotype analysis.There was statistically significant difference in the positive rate between the two methods(P<0.05).Conclusion Compared with G-banding karyotype analysis,eight-probe FISH technique has the advantages of high efficiency,time-saving,and high sensitivity.However,G-banding karyotype analysis is more extensive.The two have different emphases and complement each other to improve the detection efficiency,provide more basis for clinical individualized treatment.

关 键 词：荧光原位杂交 G显带 染色体核型分析 急性淋巴细胞白血病 

分 类 号：R733.71[医药卫生—肿瘤] R730.43[医药卫生—临床医学]