基于代谢组学初探《黄帝内经》生长发育理论的科学内涵  

Primary investigation on the theory of growth and development in Huangdi Neijing based on metabolomics

作　　者：陈文文[1] 张珏[1] 元唯安[1] 张磊阳 张芳[2] 童仁超 贺敏[1] 蒋健[1] CHEN Wen-wen;ZHANG Jue;YUAN Wei-an;ZHANG Lei-yang;ZHANG Fang;TONG Ren-chao;HE Min;JIANG Jian(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院,上海201203 [2]上海中医药大学中药研究所,上海201203

出　　处：《中华中医药杂志》2020年第3期1126-1133,共8页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市进一步加快中医药事业发展三年行动计划(No.ZY3-CCCX-3-2007);国家科技重大专项课题(No.2017ZX09304002)。

摘　　要：目的:以反映生物体整体特征的代谢组学探讨《黄帝内经》生长发育理论的科学内涵。方法:采集18~65岁健康受试者的血清和尿液样本,通过超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS)技术分析生物样本中代谢组的表达信息,运用多元统计分析方法等生物信息处理技术,研究不同性别、不同年龄段(按《内经》女七岁、男八岁划分)的血清、尿液代谢模式差异。结果:健康男性与女性的血清、尿液代谢模式存在差异;健康男性和女性各个年龄段的血清代谢模式存在差异;男性32岁前后、女性28岁前后血清和尿液代谢轮廓均显著分离,其差异主要涉及磷脂、能量、氨基酸、核苷酸等代谢过程。结论:各年龄段代谢模式存在差异,男子“四八”、女子“四七”代谢轮廓显著分离,主要涉及磷脂、能量、氨基酸、核苷酸代谢。Objective:To explore the theory of growth and development in Huangdi Neijing using metabolomics which has the characteristics of entirety and dynamics.Methods:Serum and urine samples from healthy subjects between the ages of 18 and 65 were collected and analyzed the metobolome information in serum and urine samples by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometric(UPLC-Q-TOF-MS)to find out the differences of metabolic patterns between different gender and age groups.Results:There were differences in serum and urine metabolic patterns between healthy males and females;There were differences in serum metabolic patterns between healthy males and females of all ages;The metabolic profiles of serum and urine were significantly separated before and after the ages of 32 for males and 28 for females,and the differences mainly involved phospholipid metabolism,energy metabolism,amino acid metabolism,and nucleotide metabolism.Conclusion:There are differences in metabolic patterns of different ages;The metabolic profiles are significantly separated before and after the ages of‘four by eight’for males and‘four by seven’for females,and the differences mainly involves phospholipid metabolism,energy metabolism,amino acid metabolism,and nucleotide metabolism.

关 键 词：代谢组学 代谢组 黄帝内经 生长发育理论 

分 类 号：R223[医药卫生—中医基础理论]