过表达ATG4A对晶状体上皮细胞上皮-间质转化的影响  

Effect of autophagy-related gene 4A overexpression on epithelial-mesenchymal transformation in lens epithelial cells

作　　者：闫楚凡 王静[1,2] 张劲松 YAN Chufan;WANG Jing;ZHANG Jinsong(Department of Ophthalmology,The Fourth Affiliated Hospital,China Medical University,Key Laboratory of Lens Research of Liaoning Province,Shenyang 110005,China;Cataract Centre,Shenyang Aier Excellent Eye Hospital,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第四医院眼科,辽宁省晶状体学重点实验室,沈阳110005 [2]沈阳爱尔卓越眼科医院白内障中心,沈阳110001

出　　处：《中国医科大学学报》2020年第5期391-395,共5页Journal of China Medical University

基　　金：国家自然科学基金(81870644)。

摘　　要：目的探讨自噬相关基因4A(ATG4A)过表达对晶状体上皮细胞中上皮-间质转化水平的影响。方法用细胞转染的方法在晶状体上皮细胞中过表达ATG4A并设为过表达ATG4A组(OE-ATG4A组),转染进空载体的细胞设为阴性对照组(NC组),通过实时q PCR和Western blotting方法分别检测2组细胞上皮-间质转化水平,并测定2组活性氧(ROS)水平。结果与NC组比较,OE-ATG4A组晶状体上皮细胞上皮-间质转化水平明显升高(P<0.05),但ROS水平无统计学差异(P>0.05),说明ATG4A能够促进上皮-间质转化水平,但不影响ROS水平。结论ATG4A能够促进晶状体上皮细胞中上皮-间质转化。Objective To investigate the effect of autophagy-related gene 4 A(ATG4 A)overexpression on epithelial-mesenchymal transformation(EMT)in lens epithelial cells.Methods An overexpression ATG4 A(OE-ATG4 A)group was created by transfecting ATG4 A plasmid into lens epithelial cells,and a negative control(NC)group was created by transfecting a blank vector plasmid into lens epithelial cells.To analyze EMT level in both groups,quantitative reverse transcription polymerase chain reaction and Western blotting analyses were performed.The levels of reactive oxygen species(ROS)in both groups were also evaluated.Results EMT was significantly upregulated in the OE-ATG4 A group compared to NC group(P<0.05);however,ROS levels did not statistically differ between the groups(P>0.05).Conclusion ATG4 A can promote EMT level in lens epithelial cells.

关 键 词：自噬相关基因4A 晶状体 上皮-间质转化 活性氧 

分 类 号：R776.1[医药卫生—眼科]