E2F1通过调节自噬相关蛋白表达促进卵巢癌细胞耐药  

E2F1 promotes drug resistance in ovarian carcinoma by regulating the expression of autophagy-related proteins

作　　者：李千会 陈说[1] 赵杨[1] LI Qianhui;CHEN Shuo;ZHAO Yang(Department of Gynecology,The First Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院妇科,沈阳110001

出　　处：《中国医科大学学报》2020年第5期448-453,共6页Journal of China Medical University

基　　金：中国医科大学校内科研基金新教师基金项目(XZR20160001)。

摘　　要：目的探讨E2F1在卵巢癌细胞耐药中的作用及其机制。方法通过RT-PCR及Western blotting检测顺铂耐药卵巢癌细胞系A2780/DDP及亲本A2780中E2F1 mRNA和蛋白的表达水平;在体外过表达或下调E2F1后,检测卵巢癌细胞表型和基因型。结果与亲代细胞相比,E2F1在顺铂耐药卵巢癌细胞系A2780/DDP中表达水平显著高于亲本A2780。过表达E2F1可促进顺铂耐药,上调ATG7、ATG10、LC3表达,并下调P62表达,但对ABCG2、ABCC1、ERCC1无影响。沉默E2F1可产生相反的结果。结论E2F1可通过调节自噬相关蛋白的表达促进卵巢癌细胞顺铂耐药。Objective To investigate the role of E2 F1 in drug resistance of ovarian cancer cells and its possible mechanism of action.Methods RT-PCR and Western blotting were used to detect the expression of E2 F1 mRNA and protein,respectively,in the cisplatinresistant ovarian cancer cell line A2780/DDP and parental cell line A2780.Ovarian cancer cell phenotypes and genotypes were examined in vitro after E2 F1 overexpression or downregulation.Results The expression of E2 F1 in the cisplatin-resistant ovarian cancer cell line A2780/DDP was significantly higher than that in the parental cell line A2780.Overexpression of E2 F1 promoted cisplatin resistance,upregulated ATG7,ATG10,and LC3 expression,and down-regulated P62 expression,but had no effect on ABCG2,ABCC1,and ERCC1.Silencing E2 F1 produced opposite results.Conclusion In summary,these results indicate that E2 F1 promotes cisplatin resistance in ovarian cancer by regulating the expression of autophagy-related proteins.

关 键 词：卵巢癌 E2F转录因子1 顺铂耐药 自噬 

分 类 号：R737.31[医药卫生—肿瘤]