贝母素乙诱导人肝癌SMMC-7721细胞凋亡的分子机制研究  

Research on Molecular Mechanism of the Apoptosis of Human Liver Cancer SMMC-7721 Cells Induced by Peiminine

作　　者：晁旭[1,2] 李宏[1] 王国全 董昌虎[1] 王斌 张荣强 吴洁琼[1] 张伟[1] 赵家荣[2] 张艳芳 乔菲 CHAO Xu;LI Hong;WANG Guoquan;DONG Changhu;WANG Bin;ZHANG Rongqiang;WU Jieqiong;ZHANG Wei;ZHAO Jiarong;ZHANG Yanfang;QIAO Fei(The Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China;Basic Medical School,Shaanxi University of Chinese Medicine)

机构地区：[1]陕西中医药大学第二附属医院,陕西咸阳712000 [2]陕西中医药大学基础医学院

出　　处：《西部中医药》2020年第3期26-29,共4页Western Journal of Traditional Chinese Medicine

基　　金：陕西省教育厅科学研究项目(16JK1207);陕西省中医药管理局科研项目(JCPT038,2019-ZZ-JC012)。

摘　　要：目的:探讨贝母素乙对人肝癌细胞SMMC-7721凋亡的影响及其分子机制。方法:采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium,MTT)法检测贝母素乙对人肝癌细胞SMMC-7721的抑制率;采用荧光染色和流式细胞术研究贝母素乙对细胞凋亡的影响;通过电子显微镜观察贝母素乙处理的人肝癌SMMC-7721细胞超微结构变化情况;Western blot方法检测贝母素乙处理后细胞凋亡相关蛋白的表达。结果:贝母素乙对肝癌SMMC-7721细胞的生长有显著抑制作用,24 h半数致死剂量(IC50)为0.625μg/mL。SMMC-7721细胞经不同浓度贝母素乙处理后早期凋亡和晚期凋亡细胞数量均升高。随着药物浓度的增加,细胞线粒体膜电位依次增大,SMMC-7721细胞的Caspase-3、Caspase-8和Caspase-9的活性依次升高;经贝母素乙作用后Bcl-2的蛋白表达降低,Bax蛋白表达升高;procapas-3和procapas-8的蛋白表达降低,Caspas-3和Caspas-9的表达升高。结论:贝母素乙可通过线粒体途径诱导肝癌SMMC-7721细胞凋亡。Objective:To explore the effects of peiminine on the apoptosis of human liver cancer SMMC-7721 cells and its molecular mechanism.Methods:MTT method was used to detect the inhibitory rate of peiminine on human liver cancer SMMC-7721 cells;fluorescent staining and flow cytometry to study the effects of peiminine on cellular apoptosis;electron microscope to observe ultrastructure changes of human liver cancer SMMC-7721 cells managed by peiminine;Western blot to measure the expressions of cellular apoptosis-related protein after managed by peiminine.Results:Peiminine possesses notable inhibitory effects on the growth of liver cancer SMMC-7721 cells,IC50 for 24 hours was 0.625μg/m L.After managed by different concentrations of peiminine,the numbers of SMMC-7721 cells in early and late apoptosis rose.As drug concentrations increased,cell mitochondrial membrane potential increased in turn.As drug concentrations increased,the activity of Caspase-3,Caspase-8 and Caspase-9 in SMMC-7721 cells rose in turn.After managed by peiminine,the expressions of Bcl-2 protein reduced,Bax protein expressions rose,the expressions of procapas-3 and procapas-8 lowered,the expressions of Caspas-3 and Caspas-9 lifted.Conclusion:Peiminine could induce the apoptosis of human liver cancer SMMC-7721 cells which might be through mitochondrial pathway.

关 键 词：肝癌 细胞凋亡 SMMC-7721 贝母素乙 分子机制 

分 类 号：R735[医药卫生—肿瘤]