甘薯钾离子转运蛋白HKT基因家族鉴定及其低钾胁迫下的表达模式分析  

Genome-wide identification and expression analysis of HKT family under potassium deficiency stress in sweetpotato

作　　者：靳容 胡亚亚[2] 张爱君 刘兰服[2] 刘明 韩美坤 李洪民 马志民[2] 陈晓光 唐忠厚 Jin Rong;Hu Yaya;Zhang Aijun;Liu Lanfu;Liu Ming;Han Meikun;Liu Hongmin;Ma Zhimin;Chen Xiaoguang;Tang Zhonghou(Xuzhou Institute of Agricultural Sciences in Xuhuai District/Xuzhou Sweetpotato Research Center,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology&Genetic Breeding of Sweetpotato,Ministry of Agriculture&Rural Affairs,Xuzhou 221131,Jiangsu,China;Institute of Cereal&Oil Crops,Hebei Academy of Agriculture&Forestry Sciences/Key Laboratory of Crop Genetics&Breeding of Hebei,Shijiazhuang 050035,Hebei,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所/江苏徐州甘薯研究中心/农业农村部甘薯生物学与遗传育种重点实验室,江苏徐州221121 [2]河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,河北石家庄050035

出　　处：《江苏师范大学学报（自然科学版）》2020年第1期31-36,共6页Journal of Jiangsu Normal University：Natural Science Edition

基　　金：国家重点研发计划项目(2018YFD1000704-10);国家自然科学基金面上项目(31771721);现代农业产业技术体系建设专项资金(CARS-11)。

摘　　要：为了解甘薯钾离子转运蛋白HKT基因家族的功能,分析低钾胁迫下的基因表达模式,利用甘薯(泰中6号)基因组信息,分离得到4个HKT基因家族成员,分析了基因结构、亚细胞定位、跨膜结构、系统进化树等;并研究了低钾胁迫下,徐薯22和宁紫1号2个不同耐低钾型品种的表达模式.结果显示:1)甘薯4个HKT蛋白都定位到了质膜上,具有6~7个不等的跨膜区;2)对拟南芥、水稻、玉米和甘薯等作物的HKT进化分析表明,HKTs可聚为3个进化簇,但甘薯IbHKT2和IbHKT4独自组成1个进化簇;3)IbHKT基因家族成员分布在甘薯的第3号染色体上,IbHKT1和IbHKT3、IbHKT2和IbHKT4是由于染色体片段复制导致的同源基因;4)实时定量荧光PCR分析表明,IbHKT基因受低钾胁迫诱导表达,但诱导程度在徐薯22和宁紫1号间存在差异.To understand the function of potassium ion(K^+) transport IbHKT family genes and their expression profile under K^+ deficiency stress in sweetpotato, four IbHKT genes from sweetpotato genome database(cv. Taizhong 6) were identified. The genes structure, predication of subcellular localization, transmembrane structure and phylogenetic tree were investigated. Two genotypic sweetpotatos, Xushu 32(tolerant to K^+ deficiency) and Ningzi 1(sensitive to K^+ deficiency) screened in our previous study, were used to analysis IbHKT genes expression profile under K^+ deficiency stress. The results showed that: 1)the number of transmembrane segments(TMS) ranged from 6 to 7 and all examined IbHKTs were predicted to be localized to the plasma membrane. 2)By phylogenetic analysis,HKT genes from Arabidopsis, rice, maize and sweetpotato were divided into three groups(group Ⅰ-Ⅲ), and groupⅡonly contains two genes, IbHKT2 and IbHKT4. 3)IbHKTs were located on the chromosome number #3 and segmental duplications result in two pairs of homologous genes, IbHKT1/IbHKT3 and IbHKT2/IbHKT4. 4)qRT-PCR analysis indicated that the expression level of IbHKT genes was upregulated by K^+ deficiency stress in two genotypic sweetpotatos, however the activation of IbHKTs under K^+ deficiency stress was more in Xushu 32 than that in Ningzi 1.

关 键 词：甘薯 HKT家族 基因鉴定 低钾胁迫 基因表达 

分 类 号：Q943.2[生物学—植物学] S531[农业科学—作物学]