HPLC法同时测定九龙盘中6种成分  

Simultaneous determination of six constituents in Antenoron filiforme by HPLC

作　　者：李俊 卢汝梅[1] 黎云清 梁臣艳[1] LI Jun;LU Ru mei;LI Yun qing;LIANG Chen yan(Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《中成药》2020年第5期1228-1232,共5页Chinese Traditional Patent Medicine

基　　金：广西中医药大学研究生创新项目(YCSY20190047);广西科技厅重大专项计划课题(桂科重1355001 4 1)。

摘　　要：目的 建立HPLC法同时测定九龙盘Antenoronfiliforme (Thunb) Rob.et Vaut.中没食子酸、原儿茶酸、异槲皮苷、鞣花酸、槲皮素、木犀草素的含有量.方法 九龙盘80%甲醇提取物采用COSMOSIL 5C18-PAQ色谱柱(250 mm×4.6 mm,5μm);流动相甲醇-0.2%磷酸水溶液,梯度洗脱;体积流量0.8 mL/min;双波长切换检测;柱温35℃.结果 6种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率为85.95% ~ 103.40%,RSD为0.5% ~3.0%.结论 该方法准确稳定,重复性好,可用于九龙盘的质量控制.AIM To establish an HPLC method for the simultaneous content determination of gallic acid,pro tocatechuic acid,iso quercetin,ellagic acid,quercetin and luteolin in Antenoron filiforme(Thunb)Rob.et Vaut..METHODS The analysis of 80%methyl extract of A.filiforme was performed on a 35℃thermostatic COSMOSIL 5C18 PAQ column(250 mm×4.6 mm,5μm),with the mobile phase comprising of methanol 0.2%phosphoric acid flowing at 0.8 mL/min in a gradient elution manner,and the dual wavelength switching detection was set.RESULTS Six constituents showed good linear relationships within their own ranges(r>0.9990),whose average recoveries were 85.95% 103.40%with the RSDs of 0.5% 3.0%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of A.filiforme.

关 键 词：九龙盘 没食子酸 原儿茶酸 异槲皮苷 鞣花酸 槲皮素 木犀草素 HPLC 

分 类 号：R284.1[医药卫生—中药学]