菝葜醇提物对小鼠脂肪代谢酶的影响  

Effects of Smilax china L. Ethanol Extract on Activities of Lipids Metabolism Related Enzymes in Mice

作　　者：李欣 潘永芳[1] 王娜 杨丽聪[1] 郑国栋[1] LI Xin;PAN Yong-fang;WANG Na;YANG Li-cong;ZHENG Guo-dong(Jiangxi Key Laboratory of Natural Product and Functional Food,College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学食品科学与工程学院,江西省天然产物与功能食品重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2020年第2期385-390,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(81760157);江西省研究生创新专项资金项目(2019-s175)。

摘　　要：【目的】研究菝葜醇提物(SCE)的成分及对小鼠体内脂肪代谢酶的影响。【方法】采用HPLC等方法分析SCE的成分组成。将50只雌性ICR小鼠随机分成5组,分别为对照组、高脂组、高脂添加0.25%、0.50%、1.00%SCE。投喂8周后,解剖,摘取小鼠的肝脏及腹腔内脂肪(IPAT)并称量。测定小鼠肝脏中肉毒碱转移酶(CAT)、酰基辅酶A氧化酶(ACO)、脂肪酸合成酶及磷酸腺苷激活蛋白激酶(AMPK)活性及mRNA表达量。【结果】SCE中的总多酚、总黄酮、总三萜的含量分别为:(42.54±0.45)%、(29.69±0.37)%、(14.03±0.21)%,其中落新妇苷、黄杞苷、绿原酸、白藜芦醇的含量分别是(23.65±0.36)mg/g、(7.97±0.03)mg/g、(13.65±0.19)mg/g、(7.46±0.15)mg/g。与高脂组相比,SCE喂养小鼠的体质量及体质量增加显著降低,0.50%及1.00%SCE明显减少IPAT质量。0.50%以上SCE的CAT、ACO、AMPK活性与高脂组相比显著提高。SCE明显上调CAT、ACO、AMPK的mRNA表达量。【结论】SCE中富含酚酸类化合物,可通过激活AMPK,促进脂肪酸氧化,减少脂肪沉积和体质量增加。[Objective]This study aimed to investigate the constituent of Smilax china L. ethanol extract(SCE)and effects of SCE on lipid metabolism related enzymes in high-fat diet(HFD)-fed mice.[Method]The components of SCE were analyzed by colorimetric method and high performance liquid chromatography(HPLC).Then,fifty female ICR mice were randomly divided into 5 groups and fed with diets of control,HFD,HFD+0.25%,0.50%,1.00% SCE for 8 weeks.Liver and intraperitioneal adipose tissues(IPAT)were weighted at the end of this period.The activities of carnitine acyltransferase(CAT),acyl-CoA oxidase(ACO),fatty acid synthase and AMP-activated protein kinase(AMPK)and mRNA expression level were measured.[Results]The contents of total polyphenol,total flavonoids,total triterpenoid in SCE were(42.54±0.45)%,(29.69±0.37)%,and(14.03±0.21)%,respectively.The contents of astilbin,engeletin,chlorogenic acid and resveratrol were(23.65±0.36)mg/g,(7.97±0.03)mg/g,(13.65±0.19)mg/g and(7.46±0.15)mg/g,respectively.Compared to HFD,body weight gain and IPAT were significantly reduced by the diets containing 0.50% and 1.00% SCE.Over 0.50% SCE markedly increased activities of CAT,ACO and AMPK.The mRNA expression levels of CAT,ACO and AMPK were markedly up-regulated by over 0.50% SCLE.[Conclusion]These results showed the SCE is rich in polyphenols.It might induce activation of AMPK,increase activities of CAT and ACO,promote fatty acid β-oxidation,and cause the suppressive effect on fat accumulation and body weight gain in mice.

关 键 词：菝葜醇提物 成分分析 腹腔内脂肪 脂肪代谢酶 

分 类 号：R151[医药卫生—营养与食品卫生学]