二化螟幼虫热胁迫响应的转录组分析  

Transcriptome analysis on heat tolerance of Chilo suppressalis larvae

作　　者：朱宇 刘洋 ZHU Yu;LIU Yang(Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China)

机构地区：[1]温州科技职业学院,浙江温州325006

出　　处：《浙江农业学报》2020年第5期849-857,共9页Acta Agriculturae Zhejiangensis

基　　金：浙江省教育厅一般科研项目(Y201840543)。

摘　　要：二化螟(Chilo suppressalis)对温度的适应能力很强,在高温环境下,二化螟幼虫体内能产生热激蛋白、超氧化物歧化酶和过氧化氢酶等抗氧化物质来消除氧化应激反应对其身体的伤害。本研究旨在通过高通量测序技术对分别置于38℃和26℃中2 h的二化螟幼虫进行比较转录组学分析,从转录组水平揭示短时高温胁迫对二化螟幼虫的影响。分析结果显示,所有样品的转录组序列比对到参考基因组上的序列占总体的百分比均超过89%,在参考基因组中有唯一比对位置的测序序列占总体的百分比均高于82%,有多个比对位置的序列占总体的百分比均不超过9%。通过差异表达分析得到387个差异表达基因,其中,热激蛋白和细胞色素P450这两类与二化螟热胁迫响应相关的基因为上调表达。对上调的差异基因进行KEGG富集分析,发现内质网内蛋白质加工、寿命调节通路-多物种、植物病原体相互作用和抗原处理及呈现这4条通路为显著富集。本研究揭示了二化螟幼虫热胁迫响应相关基因的总体表达特征,同时可为二化螟热胁迫响应的相关试验提供参考。Chilo suppressalis has strong adaptability to temperature.In high temperature environments,C.suppressalis larvae can produce antioxidant substances such as heat shock proteins,superoxide dismutase and catalase to eliminate the damage caused by oxidative stress.To reveal the effect of short-term high temperature on C.suppressalis larvae,a comparative transcriptome analysis between two sets of C.suppressalis larvae,treated at 38℃and 26℃for two hours respectively,was performed.The transcriptome results showed that the clean reads of all samples mapped to the reference genome accounted for more than 89%,the percentage of the sequences with unique mapped was higher than 82%,and the percentage of the sequences with multiple positions did not exceed 9%.Through differential expression analysis,387 differentially expressed genes were obtained.Among them,heat shock protein and cytochrome P450 were up-regulated expression of genes related to the response to heat stress.KEGG enrichment analysis was performed on the up-regulated differentially expressed genes,and the four pathways in the protein processing in endoplasmic reticulum,longevity regulating pathway-multiple species,plant-pathogen interaction and antigen processing and presentation were significantly enriched.This study revealed the overall expression levels of genes involved in heat stress response in the larvae of C.suppressalis,and provided references for related experiments on heat stress response of C.suppressalis.

关 键 词：二化螟 幼虫 热胁迫 转录组 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]