白术内酯Ⅰ通过调低Cyclin D1/CDK4抑制胃癌细胞SGC-7901的增殖及机制  

AtractylenolideⅠinhibit the proliferation of gastric cancer cells SGC-7901 by lowering Cyclin D1/CDK4 and its mechanism

作　　者：郭晨旭[1] 刘静波[2] 谢强 许睿 张明亮[1] 钱军[1] GUO Chen-xu;LIU Jing-bo;XIE Qiang;XU Rui;ZHANG Ming-liang;QIAN Jun(Department of Surgical Oncology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China;Department of Gynecologic Oncology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院肿瘤外科,安徽蚌埠233004 [2]蚌埠医学院第一附属医院肿瘤妇科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2020年第4期456-460,共5页Journal of Bengbu Medical College

基　　金：蚌埠医学院自然科学基金项目(BYKY1774)。

摘　　要：目的:探讨白术内酯I抑制胃癌细胞SGC-7901的增殖及可能机制。方法:MTT法检测白术内酯Ⅰ对胃癌细胞SGC-7901增殖的抑制作用;流式细胞仪检测白术内酯Ⅰ作用后胃癌细胞SGC-7901的凋亡率及细胞周期的改变;Western blotting检测度白术内酯Ⅰ作用后胃癌细胞SGC-7901中Cyclin D1、CDK4蛋白的变化。结果:MTT试验结果显示与对照组相比,白术内酯Ⅰ可抑制胃癌细胞SGC-7901的增殖,并且呈时间及剂量的依赖性(P <0. 05~P <0. 01);流式细胞仪结果显示与对照组相比白术内酯Ⅰ能够明显促进胃癌细胞SGC-7901后的细胞凋亡,并且呈剂量的依赖性(P <0. 01),同时会增加细胞中G1期细胞的比例(P <0. 01),减少G2期细胞的比例(P <0. 01),并且呈剂量的依懒性(P <0. 05);Western blotting结果显示同对照组相比白术内酯Ⅰ能够调低胃癌细胞SGC-7901中Cyclin D1、CDK4蛋白的表达(P <0. 01),并且呈剂量的依赖性(P <0. 05)。结论:白术内酯Ⅰ能通过调低Cyclin D1、CDK4蛋白进而改变细胞周期来抑制胃癌细胞SGC-7901的增殖同时促进其凋亡。Objective:To investigate the inhibition of atractylenolideⅠon the proliferation of gastric cancer cell line SGC-7901 and its possible mechanism.Methods:After the atractylenolideⅠtreatment,the inhibitory effects of atractylenolideⅠon the proliferation of gastric cancer cells SGC-7901 were detected using MTT assay,the apoptosis rate and cell cycle of gastric cancer cells SGC-7901 were detected using flow cytometry,and the changes of Cyclin D1 and CDK4 proteins in gastric cancer cells SGC-7901 were detected using Western blotting.Results:The results of MTT assay showed that atractylenolideⅠcould inhibit the proliferation of gastric cancer cells SGC-7901 in a time and dose-dependent manner compared with the control group(P<0.05 to P<0.01).The results of flow cytometry showed that the atractylenolideⅠcould significantly promote the apoptosis of gastric cancer cells SGC-7901(P<0.01),increase the ratio of G 1 phase cells(P<0.01),and decrease the ratio of G 2 phase cells in a dose-dependent manner compared with the control group(P<0.01).The results of Western blotting showed that atractylenolideⅠcould reduce the expression levels of Cyclin D1 and CDK4 proteins in SGC-7901 cells in a dose-dependent manner compared with the control group(P<0.01).Conclusions:AtractylenolideⅠcan inhibit the proliferation,and promote apoptosis of gastric cancer cells SGC-7901 by reducing the levels of Cyclin D1 and CDK4 protein to change cell cycle.

关 键 词：胃肿瘤 白术内酯Ⅰ 增殖 凋亡 细胞周期蛋白D1 CDK4蛋白 

分 类 号：R735.2[医药卫生—肿瘤]