PD-L1和IFN-γ与系统性红斑狼疮患者免疫功能的关系  

Research on relationship between PD-L1,IFN-γand immune function in patients with systemic lupus erythematosus

作　　者：孟芳[1] 廖永强[1] 肖妮 戴森华[2] 夏洪娇[1] 陈波[1] MENG Fang;LIAO Yongqiang;XIAO Ni;无(PingXiang People’s Hospital,clinical laboratory,PingXiang 337000,China;Department of rheumatology,PingXiang,337000,China)

机构地区：[1]江西省萍乡市人民医院检验科,萍乡337000 [2]江西省萍乡市人民医院风湿免疫科,萍乡337000

出　　处：《江西医药》2020年第5期510-513,共4页Jiangxi Medical Journal

基　　金：江西省萍乡市科技计划项目(培育类),编号2018PY037。

摘　　要：目的探讨SLE患者血清PD-L1与IFN-γ及它们与免疫功能的关系。方法选取我院2018年1月-2019年12月住院及门诊部诊治的SLE患者50例(SLE组)和健康体检者45例(对照组),根据SLE疾病活动指数(systemic lupus erythematosus disease activity index,SLEDAI)将SLE组分为SLE活动组和非活动组(分别为18例,32例)。血清PD-L1和IFN-γ水平检测采用ELISA法,细胞表面PD-L1表达检测采用流式细胞术,收集疾病活动性指标和相关实验室数据。结果⑴与健康对照组相比,SLE患者外周血CD4+T细胞表面PD-L1分子表达上调,且活动组显著高于非活动组(P<0.01);CD8+T细胞和CD19+B细胞表面PD-L1分子表达下调,且活动组显著低于非活动组(P<0.01);⑵活动组SLE患者PD-L1,补体C3和C4血清水平显著低于非活动组和健康对照组(均P<0.01),而IFN-γ,CRP、SLEDAI及抗dsDNA抗体滴度显著高于非活动组和健康对照组(均P<0.01);⑶SLE患者PD-L1血清水平与IFN-γ、SLEDAI、抗dsDNA抗体滴度和CRP呈显著负相关(r=-0.428,-0.434,-0.453,-0.362,均P<0.05),与补体C3和C4呈显著正相关(r=0.438,0.345,均P<0.05)。结论在SLE患者体内,PD-L1和IFN-γ异常表达于T、B淋巴细胞表面和血清,一起参与免疫功能的调节。Objective To evaluate the relationship between PD-L1,IFN-γand immune function in SLE patients.Methods Fifty SLE patients diagnosed at our hospital from january 2018 to december 2019 were included in the study.Additionally,forty-five people coming for healthy examination during the same period were grouped into control.The SLE group was further divided into two groups according to systemic lupus erythematosus disease activity index(SLEDAI):activity group and none activity group(n=32,18).Serum Levels of PD-L1 and IFN-γwere detected by ELISA,PD-1 and PD-L1 expression on cell surface was detected by flow cytometry,the laboratory data related to disease activity were collected during the same period.Results(1)Compared with healthy person,expression quantity of PD-L1 on the surface of CD4+T cells in SLE patients was up-regulated,and activity group was higher than none activity(P<0.01).The expression of PD-1on the surface of CD8+T cells and CD19+B cells was up-regulated,and activity group was higher than none activity(P<0.01),while the expression of PD-L1 was down regulated,and the activity group was lower than none activity(P<0.01).(2)The serum level of PD-L1,complement C3 and C4 in activity SLE patients was significantly lower than none activityand the healthy controls(P<0.01),while level of IFN-γ,CPR,SLEDAI andanti-dsDNA antibody titer was significantly higher than none activity and healthy controls(P<0.01).(3)The serum level of PD-L1 was negatively correlated with IFN-γ,SLEDAI,anti-dsDNA antibody titer and CRP(r=-0.428,-0.434,-0.453,-0.362,P<0.05),positively with complement C3 and C4(r=0.438,0.345,P<0.05).Conclusion PD-L1 and IFN-γare abnormally expressed on the surface of T,B lymphocytes and in serum,to participate in the regulation of immune function together.

关 键 词：系统性红斑狼疮 免疫功能 PD-L1 IFN-Γ 

分 类 号：R593.241[医药卫生—内科学]