基于18S rRNA基因对植物寄生线虫的系统学研究  

Phylogenetic Studies of Plant Parasitical Nematodes Based on 18S rRNA Gene

作　　者：李戌清[1,2] 郑经武[1] LI Xu-Qing;ZHENG Jing-Wu(College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China)

机构地区：[1]浙江大学农业与生物技术学院,杭州310058 [2]杭州市农业科学研究院,杭州310024

出　　处：《农业生物技术学报》2020年第4期731-742,共12页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31371921)。

摘　　要：长期以来,植物线虫的鉴定和分类主要以形态学特征和测量数据为依据,有关的方法主观且繁琐,难以可靠地鉴别线虫种内和种间群体间差异,而以DNA为基础的分子分类学研究方法,提供的信息客观简便,更能揭示不同类群的线虫在进化过程中的系统学关系。本研究分别利用NEMF1/S3和A/S3两对引物对来源于国内外植物根围土壤的小杆目、矛线目和三矛目94个植物寄生线虫种群的18S rRNA基因进行PCR扩增,所得序列经DNAstar软件联配比较、编辑,并用MEGA 6.0软件进行系统发育分析,以最大似然法(maximum likelihood, ML)构建系统发育树,结果发现,基于引物对A/S3扩增序列构建的植物线虫系统发育树能系统体现出不同线虫类群及同一线虫类群内不同种群间的系统发育关系。相关研究结果将为未来植物线虫系统进化研究提供一定的借鉴。Traditional methods for identification and classification of plant nematodes are mainly based on morphological characters and morphometrics. These methods are subjective and cumbersome, and are difficult to make reliably taxonomy and identification. The molecular method for taxonomy and identification based on DNA not only provides objective information, but also can reveal the evolution of different groups of nematodes. In this study, 18 S r RNA gene fragments of 94 plant parasitic nematode populations, which belonging to Rhabditida, Dorylaimida and Triplonchida, were amplified with primer sets NEMF1/S3 and A/S3, respectively. The sequences were compared and edited by DNAstar software, and were used to construct the phylogenetic trees by Mega 6.0 software. The results showed that the phylogenetic tree constructed based on the sequences, which PCR amplified with primers A/S3, can reflect the phylogenetic relations of nematode species within different groups and the same group. The results provide reference to study phylogeny of plant nematodes in the future.

关 键 词：植物寄生线虫 18S RRNA 小杆目 矛线目 膜皮目 系统分析 

分 类 号：S432.45[农业科学—植物病理学] Q959.17[农业科学—农业昆虫与害虫防治]